Donkey Anti-Mouse IgG H&L (TRITC) (ab6817)
Key features and details
- Donkey Anti-Mouse IgG H&L (TRITC)
- Conjugation: TRITC. Ex: 547nm, Em: 572nm
- Host species: Donkey
- Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA
Related conjugates and formulations
Overview
-
Product name
Donkey Anti-Mouse IgG H&L (TRITC)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Mouse -
Tested applications
Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details -
Immunogen
Mouse IgG whole molecule
-
Conjugation
TRITC. Ex: 547nm, Em: 572nm
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate -
Concentration information loading...
-
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Immunomicroscopy |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use at an assay dependent dilution.
|
|
ICC/IF |
1/1000 - 1/5000.
|
|
IHC-P |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
|
ELISA |
1/10000 - 1/50000.
|
Notes |
---|
Immunomicroscopy
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent dilution. |
ICC/IF
1/1000 - 1/5000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ELISA
1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab6817 has been referenced in 3 publications.
- Li M et al. Functional Interactions Between the Parafascicular Thalamic Nucleus and Motor Cortex Are Altered in Hemiparkinsonian Rat. Front Aging Neurosci 14:800159 (2022). PubMed: 35677204
- Wang X et al. Electrophysiological and Neurochemical Considerations of Distinct Neuronal Populations in the Rat Pedunculopontine Nucleus and Their Responsiveness Following 6-Hydroxydopamine Lesions. Front Neurosci 13:1034 (2019). PubMed: 31616246
- Tao X et al. In vitro examination of microglia-neuron crosstalk with BV2 cells, and primary cultures of glia and hypothalamic neurons. Heliyon 4:e00730 (2018). PubMed: 30148218