Overview

  • Product name

    Donkey Anti-Rabbit IgG H&L (10nm Gold)
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Rabbit
  • Tested applications

    Suitable for: Electron Microscopymore details
  • Immunogen

    Rabbit IgG whole molecule.

  • Conjugation

    Gold 10nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    This product is an immunogold reagent built around colloidal gold particles of 10nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 10nm particle size conjugate is less than 10 %.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab39597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notes
    Electron Microscopy: Use at an assay dependent dilution.
    Light Microscopy: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    This product has been referenced in:

    • Müller WEG  et al. Polyphosphate as a donor of high-energy phosphate for the synthesis of ADP and ATP. J Cell Sci 130:2747-2756 (2017). Read more (PubMed: 28687622) »
    • Goupil LS  et al. Cysteine and Aspartyl Proteases Contribute to Protein Digestion in the Gut of Freshwater Planaria. PLoS Negl Trop Dis 10:e0004893 (2016). Read more (PubMed: 27501047) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Question
    Answer

    Le secondaire ab39597 couplé aux billes d'or de 10nm a les caratéristiques suivantes :

    3.3 x 1011 particules d'or / ml
    115-180 anticorps / bille d'or
    La concentration d'anticorps est d'environ 10 µg/ml

    Read More

    Question
    Answer


    1. ces réactifs n'ont pas de concentration déterminée mais ils sont fournis à une DO de 1.0 à 520nm,
    2. la concentration en anticorps est comprise entre 5 et 10 µg/ml,
    3. Une particule d'or de 10nm possède entre 7 et 10 molécules d'anticorps à sa surface,
    4. la dilution recommandée est de 1/20.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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