Key features and details
- Donkey Anti-Rabbit IgG H&L (25nm Gold)
- Conjugation: Gold 25nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: Electron Microscopy
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.097% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system [Moeremans et al., J. Immunol., Methods 74, (1984), 353.]
This product is an immunogold reagent built around colloidal gold particles of 25nm. The particle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coeffiecient of variance for the 25nm particle size conjugate is less than 12%.
Our Abpromise guarantee covers the use of ab41514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Light Microscopy: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab41514 has been referenced in 1 publication.
- Rossi A et al. Identification and dynamic changes of RNAs isolated from RALY-containing ribonucleoprotein complexes. Nucleic Acids Res 45:6775-6792 (2017). PubMed: 28379492