For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    donkey-rabbit-igg-hl-6nm-gold-preadsorbed-ab105294.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Immunoglobulins Heavy Chain IgG
Share by email

Donkey Anti-Rabbit IgG H&L (6nm Gold) preadsorbed (ab105294)

  • Datasheet
  • SDS
Submit a review Q&A (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Donkey Anti-Rabbit IgG H&L (6nm Gold) preadsorbed
  • Conjugation: Gold 6nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: Electron Microscopy, WB, ELISA

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 25nm HRP IRDye® 680RD Texas Red ® TRITC Unconjugated Unconjugated

You may also be interested in

Biochemical
Product image
Losartan potassium, non-peptide angiotensin II AT1 receptor antagonist (ab120997)
Secondary
Goat Anti-Mouse IgG2a heavy chain (PE/Cy7®) preadsorbed (ab130787)
Primary
Product image
HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)

View more associated products

Overview

  • Product name

    Donkey Anti-Rabbit IgG H&L (6nm Gold) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Rabbit
  • Specificity

    Recognizes the heavy chains on rabbit IgG and the light chains common to most rabbit immunoglobulins. Does not crossreact with non-immunoglobulin serum proteins. Exhibits minimal crossreactivity to bovine, chicken, goat, guinea pig, syryan hamster, horse, human, mouse, rat and sheep serum proteins.
  • Tested applications

    Suitable for: Electron Microscopy, WB, ELISAmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Horse, Human, Mouse, Rat, Sheep, Syrian hamster
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Conjugation

    Gold 6nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 8.50
    Preservative: 0.05% Sodium azide
    Constituents: 0.164% Sodium phosphate, 0.381% Sodium borate, 1.5% BSA, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Gold

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab105294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
1/20 - 1/40.
WB
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Notes
Electron Microscopy
1/20 - 1/40.
WB
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab105294? Please let us know so that we can cite the reference in this datasheet.

ab105294 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

Do you have an EM protocol for use of the antibody?
When are the primary and secondary antibodies added? Before or after the cells are fixed for EM?

Many thanks

Read More

Abcam community

Verified customer

Asked on Mar 01 2013

Answer

Fixation precedes incubation with primary and secondary antibodies.

The protocol for gold-antibody complexes is essentially the same as for all conjugates with the exception of the buffer used to dilute and wash.


Step 1: Block with 5% normal serum from the host species of the gold-secondary antibody diluted in PBS.

Step 2: Wash with PBS-BSA.

In each step the grid is floated on a drop of solution.



Control: Step 3: Incubate directly with the secondary antibody without addition of the primary antibody.


Step 4: Wash with PBS-BSA.

This control experiment will tell you if the secondary is giving background. If it is not giving background but there is background in the full experiment, you know that any background is coming from the primary.



Full Experiment: Step 3: Incubate with the primary antibody diluted in PBS-BSA.

Step 4: Wash with PBS-BSA.

Step 5: Incubate with the gold labeled secondary antibody.

Step 6: Wash with PBS-BSA

Read More

Abcam Scientific Support

Answered on Mar 01 2013

Question

Konzentration? Verduennung fuer EM nicht ausrechenbar.

Read More

Abcam community

Verified customer

Asked on Mar 07 2012

Answer

Vielen Dank für Ihren Anruf.

Wie versprochen, habe ich das Labor wegen der genauen Konzentration von ab105294 kontaktiert, und habe erfahren, dass die genaue Konzentration leider nicht bestimmt werden kann. Zwar wird der Antikörper vor der Konjugation mit den Goldkomplexen aufgereinigt. Die exakte Konzentration lässt sich jedoch nicht bestimmen, weil nach der Konjugation Monomere von Multimeren über eine Dichtegradientenzentrifugation abgetrennt werden. Diese Monomere werden vor und nach der Zentrifugation mit Albumin stabilisiert, und leider gibt es keine verlässliche Methode, die Konzentration des Antikörpers in dieser Präparation zu bestimmen.

Nach unserer Erfahrung zeigt dieser sekundäre Antikörper in der EM in Verdünnungen von 1:10 bis 1:100 gute Ergebnisse, so dass wir hoffen, diese Angaben können Ihnen für Ihre Experimente als Anhaltspunkt dienen.

Ich möchte mich dafür entschuldigen, dass ich nicht mehr Informationen für Sie habe und hoffe, dass diese Angabe dennoch hilfreich für Sie ist. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Read More

Abcam Scientific Support

Answered on Mar 07 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.