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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (ab175648)

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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (ab175648)
  • Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (ab175648)

Key features and details

  • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed
  • Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt

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Overview

  • Product name

    Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Rabbit
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.

     

  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • Minimal
    cross-reactivity


    Human, Mouse, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 405. Ex: 402nm, Em: 421nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Fluorophore
    • Alexa Fluor® 405

Associated products

    Applications

    Our Abpromise guarantee covers the use of ab175648 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr Use at an assay dependent concentration.
    ICC/IF 1/200 - 1/1000.

    We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below)
    Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm
    Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm

    ELISA Use at an assay dependent concentration.
    IHC-P Use at an assay dependent concentration.
    Flow Cyt 1/2000.

    Images

    • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (ab175648)
      Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (ab175648)

      ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (blue) was ab175648 Alexa Fluor® 405 Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L, used at 1µg/ml for 1h. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25µM.

      The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

    • Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (ab175648)
      Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed (ab175648)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab175648? Please let us know so that we can cite the reference in this datasheet.

    ab175648 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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