Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
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- Specific References (41)
- Protocols
Overview
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Product nameDonkey Anti-Rabbit IgG H&L (Alexa Fluor® 488)
See all IgG secondary antibodies -
DescriptionDonkey polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488)
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Host speciesDonkey
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Target speciesRabbit
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SpecificityThis antibody is specific to Rabbit IgG
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Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-P, ELISA, IHC-Frmore details
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Immunogen
The details of the immunogen for this antibody are not available.
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ConjugationAlexa Fluor® 488. Ex: 495nm, Em: 519nm
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
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Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
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ClonalityPolyclonal
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IsotypeIgG
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
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Research areas
Associated products
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Related Products
Applications
Our Abpromise guarantee covers the use of ab150073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| ICC/IF | 1/200 - 1/1000. | |
| Flow Cyt | 1/2000 - 1/4000. | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
Images
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)ICC/IF image of ab6046 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab150073 Alexa Fluor® 488 donkey anti-rabbit IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10min).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)This image is courtesy of an Abreview submitted by Laura Comley.ab104603 staining DYNLL1 in mouse cervical spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with 10% serum for 1 hour at 21°C. Samples were incubated with primary antibody (2 µg/ml in PBS/10% serum/0.1% Triton X-100) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG H&L (ab150073) (1/500) was used as the secondary antibody.
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Flow Cytometry - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)Overlay histogram showing Jurkat cells stained with ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal donkey serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Donkey anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150073) was used at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
References
This product has been referenced in:
- Larasati YA et al. Curcumin targets multiple enzymes involved in the ROS metabolic pathway to suppress tumor cell growth. Sci Rep 8:2039 (2018). Read more (PubMed: 29391517) »
- Li C et al. Nfatc1 Is a Functional Transcriptional Factor Mediating Nell-1-Induced Runx3 Upregulation in Chondrocytes. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29316655) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"