Application
Western blot
Application Western blot
Sample Mouse Rat lysate - Cytoplasm
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s)· Concentration: 5% · Temperature: 25°C
Other product details
Primary antibody- ab17820 Rabbit Polyclonal to Caspase 1
Dilution 1/1000, 1/2000. 1/5000
Incubation time 24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Application Western blot
Dilution 1/1000, 1/2000, 1/5000
Detection
Biorad Gel Doc XR+
Bands Specific: 45, 20 kDa
Stained membrane to check for protein transfer, all looked good. Incubated in both primary and secondary antibody and imaged, no bands were present at any of the dilutions.
Sample Mouse Rat lysate - Cytoplasm
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s)· Concentration: 5% · Temperature: 25°C
Other product details
Primary antibody- ab17820 Rabbit Polyclonal to Caspase 1
Dilution 1/1000, 1/2000. 1/5000
Incubation time 24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Application Western blot
Dilution 1/1000, 1/2000, 1/5000
Detection
Biorad Gel Doc XR+
Bands Specific: 45, 20 kDa
Stained membrane to check for protein transfer, all looked good. Incubated in both primary and secondary antibody and imaged, no bands were present at any of the dilutions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Tammie Quinn
Verified customer
Submitted Sep 26 2013