Nice, bright clear staining seen using secondary antibody at a 1:200 dilution. Primary antibody used here was anti-Nanog (ab21624). Small amounts of speckled areas noted.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Sarah Ritson
Submitted Oct 07 2013