Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed (ab150066)
Key features and details
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, ELISA, Flow Cyt, IHC-Fr
Overview
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Product name
Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, ELISA, Flow Cyt, IHC-Frmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Human, Mouse, Pig, Rat more details -
Immunogen
The details of the immunogen for this antibody are not available.
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Conjugation
Alexa Fluor® 555. Ex: 555nm, Em: 565nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/200 - 1/1000.
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IHC-P |
Use at an assay dependent concentration. Use at an assay dependent dilution
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ELISA |
Use at an assay dependent concentration. Use at an assay dependent dilution
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Flow Cyt |
1/2000.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
1/200 - 1/1000. |
IHC-P
Use at an assay dependent concentration. Use at an assay dependent dilution |
ELISA
Use at an assay dependent concentration. Use at an assay dependent dilution |
Flow Cyt
1/2000. |
IHC-Fr
Use at an assay dependent concentration. |
Images
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ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150066 Alexa Fluor® 555 donkey anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab150066 Alexa Fluor® 555 donkey anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab150066 has been referenced in 14 publications.
- Li D et al. Abnormal lipid droplets accumulation induced cognitive deficits in obstructive sleep apnea syndrome mice via JNK/SREBP/ACC pathway but not through PDP1/PDC pathway. Mol Med 28:3 (2022). PubMed: 35030992
- Permanne B et al. O-GlcNAcase Inhibitor ASN90 is a Multimodal Drug Candidate for Tau and α-Synuclein Proteinopathies. ACS Chem Neurosci 13:1296-1314 (2022). PubMed: 35357812
- Baek SM et al. Senescence Marker Protein 30 (SMP30): A Novel Pan-Species Diagnostic Marker for the Histopathological Diagnosis of Breast Cancer in Humans and Animals. Int J Mol Sci 22:N/A (2021). PubMed: 33652881
- Hinteregger B et al. Metabolic, Phenotypic, and Neuropathological Characterization of the Tg4-42 Mouse Model for Alzheimer's Disease. J Alzheimers Dis 80:1151-1168 (2021). PubMed: 33646155
- Ullah HMA et al. Nogo-A Is Critical for Pro-Inflammatory Gene Regulation in Myocytes and Macrophages. Cells 10:N/A (2021). PubMed: 33572505