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    donkey-rabbit-igg-hl-alexa-fluor-568-ab175470.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (34)

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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470)
  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470)
  • ELISA - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470)
  • Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470)

Key features and details

  • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568)
  • Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, IHC-P, Flow Cyt, ELISA

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 25nm Gold 6nm HRP IRDye® 680RD Texas Red ® TRITC Unconjugated Unconjugated

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Overview

  • Product name

    Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568)
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, IHC-P, Flow Cyt, ELISAmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 568. Ex: 578nm, Em: 603nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Fluorophore
    • Alexa Fluor® 568

Associated products

  • Alternative Versions

    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150074)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150076)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175651)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) (ab175731)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175772)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175780)
    • Donkey Anti-Rabbit IgG H&L (ab182020)
    • Donkey Anti-Rabbit IgG H&L (HRP) (ab205722)
    • Donkey Anti-Rabbit IgG H&L (Biotin) (ab207999)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
IHC-P (1)
Use at an assay dependent concentration.
Flow Cyt (1)
1/2000.
ELISA
Use at an assay dependent concentration.
Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
1/2000.
ELISA
Use at an assay dependent concentration.

Images

  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470)
    Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470)

    ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1% BSA/10% normal donkey serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175470, Alexa Fluor® 568 Donkey anti-Rabbit IgG - H&L, used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Alexa Fluor® 568 is pseudocolored in yellow.

  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470)
    Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470)
    HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab175470, Alexa Fluor® 568 Donkey anti-Rabbit IgG - H&L, used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

     

    Alexa Fluor® 568 is pseudocolored in yellow.

  • ELISA - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470)
    ELISA - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470)

    Cross-reactivity of the polyclonal secondary antibody ab182020 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182020 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Goat anti-Donkey IgG H&L (HRP) (ab6988) was used at 1/20,000 dilution (50 µl/well), followed by incubation for 1h at RT.

    For the batch tested, ab182020 showed a cross-reactivity below 2% towards human IgG, mouse IgG, rat IgG, goat IgG and chicken IgY.

    This data was developed using the unconjugated antibody (ab182020).

  • Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470)
    Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (34)

Publishing research using ab175470? Please let us know so that we can cite the reference in this datasheet.

ab175470 has been referenced in 34 publications.

  • Cooler S & Schwartz GW An offset ON-OFF receptive field is created by gap junctions between distinct types of retinal ganglion cells. Nat Neurosci 24:105-115 (2021). PubMed: 33230322
  • Chaumonnot K  et al. The HSP GRP94 interacts with macrophage intracellular complement C3 and impacts M2 profile during ER stress. Cell Death Dis 12:114 (2021). PubMed: 33483465
  • Teafatiller T  et al. Upregulation of Vitamin C Transporter Functional Expression in 5xFAD Mouse Intestine. Nutrients 13:N/A (2021). PubMed: 33672967
  • Ouyang J  et al. Neuroligin1 Contributes to Neuropathic Pain by Promoting Phosphorylation of Cofilin in Excitatory Neurons. Front Mol Neurosci 14:640533 (2021). PubMed: 33716669
  • Orfanou IM  et al. Discovery and Pharmacological Evaluation of STEAP4 as a Novel Target for HER2 Overexpressing Breast Cancer. Front Oncol 11:608201 (2021). PubMed: 33842315
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Good secondary

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
This secondary ab works as expected. I used the dilution 1in1000.
The picture shows a picture where mouse cells (engineered to express mCherry) were stained with the primary antibody for mCherry (ab213511) and subsequently stained with ab175470.

The picture was taken with a Leica Microscope using a 20X objective.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Francesco Elia Marino

Verified customer

Submitted Sep 26 2018

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