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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175692)

  • Datasheet
Reviews (1) Submit a question References (15)

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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175692)
  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175692)
  • Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175692)

Key features and details

  • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed
  • Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-Fr, IHC-P, ELISA, ICC/IF, Flow Cyt

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Overview

  • Product name

    Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Rabbit
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.

     

  • Tested applications

    Suitable for: IHC-Fr, IHC-P, ELISA, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity


    Human, Mouse, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 568. Ex: 578nm, Em: 603nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Fluorophore
    • Alexa Fluor® 568

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
Flow Cyt
1/2000.
Notes
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
Flow Cyt
1/2000.

Images

  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175692)
    Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175692)

    ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175692, Alexa Fluor® 568 Donkey anti-Rabbit IgG - H&L, used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

     

    Alexa Fluor® 568 is pseudocolored in yellow.

  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175692)
    Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175692)
    HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab175692, Alexa Fluor® 568 Donkey anti-Rabbit IgG - H&L, used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

     

    Alexa Fluor® 568 is pseudocolored in yellow.

  • Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175692)
    Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175692)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (15)

Publishing research using ab175692? Please let us know so that we can cite the reference in this datasheet.

ab175692 has been referenced in 15 publications.

  • Zheng X  et al. Host interneurons mediate plasticity reactivated by embryonic inhibitory cell transplantation in mouse visual cortex. Nat Commun 12:862 (2021). PubMed: 33558487
  • Poon C  et al. Neutrophil recruitment and leukocyte response following focused ultrasound and microbubble mediated blood-brain barrier treatments. Theranostics 11:1655-1671 (2021). PubMed: 33408773
  • Chung Nien Chin S  et al. Coordinate expression loss of GKN1 and GKN2 in gastric cancer via impairment of a glucocorticoid-responsive enhancer. Am J Physiol Gastrointest Liver Physiol 319:G175-G188 (2020). PubMed: 32538140
  • Ozen M  et al. Magnesium sulfate inhibits inflammation through P2X7 receptors in human umbilical vein endothelial cells. Pediatr Res 87:463-471 (2020). PubMed: 31493768
  • Kaufman AM  et al. A Role for the Locus Coeruleus in Hippocampal CA1 Place Cell Reorganization during Spatial Reward Learning. Neuron 105:1018-1026.e4 (2020). PubMed: 31980319
View all Publications for this product

Customer reviews and Q&As

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Excellent

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA fixed)
Stain PFA-fixed, free-floating section of mouse. The concentration is 1:250.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Lin Lin

Verified customer

Submitted Jul 25 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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