Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (ab150067)


  • Product name

    Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed
    See all IgG secondary antibodies
  • Host species

  • Target species

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.


  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, ELISA, Flow Cyt, IHC-Pmore details
  • Minimal

    Chicken, Cow, Goat, Human, Pig, Rat more details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

  • Isotype

  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or

  • Research areas


Our Abpromise guarantee covers the use of ab150067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
ELISA Use at an assay dependent concentration.
Flow Cyt 1/2000.

ab199093 - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control to complement this secondary antibody.


IHC-P Use at an assay dependent concentration.


  • ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (red) was ab150067 Alexa Fluor® 647 donkey anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Gustavsson AK  et al. 3D single-molecule super-resolution microscopy with a tilted light sheet. Nat Commun 9:123 (2018). WB ; Human . Read more (PubMed: 29317629) »
  • Lautenschläger J  et al. C-terminal calcium binding of a-synuclein modulates synaptic vesicle interaction. Nat Commun 9:712 (2018). Read more (PubMed: 29459792) »
See all 4 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (PFA fixed)
Rat brain (28 d post-stroke) was perfusion-fixed using 4% PFA and cryo-sectioned in the coronal plane (20 µm thick).
Primary antibody: rabbit anti-GFAP antibody (Sigma-Aldrich)
Secondary antibody: Donkey anti-rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (Abcam ab150067)
Blocking buffer: 10% Donkey serum, 3% BSA, 0.3% Triton X-100 in PBS (pH 7.4)
Brain section was blocked for 2 h at room temperature.
Incubation in primary antibody diluted in blocking buffer (1:400 dilution) at 4 °C for 16-18 h.
Incubation in secondary antibody diluted in PBS (1:2000 dilution) at room temperature for 2 h.
Image (scale bar = 200 µm) shown is taken within the peri-infarct region where GFAP expression is upregulated.
Strong fluorescent signal with low background noise can be observed.

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Submitted Nov 09 2016

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