Product nameDonkey Anti-Rabbit IgG H&L (Alexa Fluor® 750)
See all IgG secondary antibodies
SpecificityThis antibody is specific to Rabbit IgG
Tested applicationsSuitable for: WBmore details
The details of the immunogen for this antibody are not available.
ConjugationAlexa Fluor® 750. Ex: 749nm, Em: 775nm
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
We batch test Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750),ab175731 in fluorescent WB. Although we don’t batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success usingDonkey Anti-Rabbit IgG H&L (Alexa Fluor® 750), ab175731 in these applications.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150074)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150076)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175651)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175772)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175780)
- Donkey Anti-Rabbit IgG H&L (ab182020)
- Donkey Anti-Rabbit IgG H&L (HRP) (ab205722)
- Donkey Anti-Rabbit IgG H&L (Biotin) (ab207999)
Our Abpromise guarantee covers the use of ab175731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Anti-beta Tubulin antibody - Loading Control (ab6046) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) (ab175731) at 1/10000 dilution
Observed band size: 50 kDa why is the actual band size different from the predicted?
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6046 overnight at 4°C. Antibody binding was detected using ab175731 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Cross-reactivity of the polyclonal secondary antibody ab182020 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182020 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Goat anti-Donkey IgG H&L (HRP) (ab6988) was used at 1/20,000 dilution (50 µl/well), followed by incubation for 1h at RT.
For the batch tested, ab182020 showed a cross-reactivity below 2% towards human IgG, mouse IgG, rat IgG, goat IgG and chicken IgY.
This data was developed using the unconjugated antibody (ab182020).
This product has been referenced in:
- Benoit A et al. Flow cytometry for receptor analysis from ex-vivo brain tissue in adult rat. J Neurosci Methods 304:11-23 (2018). Flow Cyt . Read more (PubMed: 29660368) »
- Zou Y et al. Notch 2 signaling contributes to cell growth, anti-apoptosis and metastasis in laryngeal squamous cell carcinoma. Mol Med Rep 14:3517-24 (2016). WB . Read more (PubMed: 27572051) »