Product nameDonkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed
See all IgG secondary antibodies
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.
Tested applicationsSuitable for: WBmore details
Human, Mouse, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
The details of the immunogen for this antibody are not available.
ConjugationAlexa Fluor® 750. Ex: 749nm, Em: 775nm
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
We batch test Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed, ab175728 in fluorescent WB. Although we don’t batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed, ab175728 in these applications.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
Our Abpromise guarantee covers the use of ab175728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Anti-beta Tubulin antibody - Loading Control (ab6046) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) preadsorbed (ab175728) at 1/10000 dilution
Observed band size: 50 kDa why is the actual band size different from the predicted?
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6046 overnight at 4°C. Antibody binding was detected using ab175728 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
ab175728 has not yet been referenced specifically in any publications.