Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6803)
Key features and details
- Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Donkey
- Suitable for: WB, ELISA, Dot blot, ICC/IF, IHC-P, IHC-Fr
Overview
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Product name
Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Tested applications
Suitable for: WB, ELISA, Dot blot, ICC/IF, IHC-P, IHC-Frmore details -
Immunogen
Rabbit IgG whole molecule
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons). -
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2500.
|
|
ELISA |
Use at an assay dependent concentration.
|
|
Dot blot |
Use at an assay dependent concentration.
|
|
ICC/IF |
1/200 - 1/1000.
|
|
IHC-P |
1/200 - 1/1000.
|
|
IHC-Fr |
1/200 - 1/1000.
|
Notes |
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WB
1/500 - 1/2500. |
ELISA
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
ICC/IF
1/200 - 1/1000. |
IHC-P
1/200 - 1/1000. |
IHC-Fr
1/200 - 1/1000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab6803 has been referenced in 1 publication.
- Sun Q et al. C-X-C-Chemokine-Receptor-Type-4 Inhibitor AMD3100 Attenuates Pulmonary Inflammation and Fibrosis in Silicotic Mice. J Inflamm Res 15:5827-5843 (2022). PubMed: 36238768