Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
Tested applications
Suitable for: ICC, ELISA, WB, IHC-Pmore details -
Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.10
Preservative: 0.09% Sodium azide
Constituents: 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP). -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Part of the AbExcel range. -
Research areas
Applications
Our Abpromise guarantee covers the use of ab97061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use at an assay dependent dilution. | |
| ELISA | 1/5000 - 1/50000. | |
| WB | 1/5000 - 1/50000. | |
| IHC-P | 1/100 - 1/1000. |
Images
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Western blot - Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061)All lanes : Anti-beta Actin antibody (ab8227) at 1 µg/ml
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061) at 1/5000 dilution
Lanes 3-4 : Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061) at 1/20000 dilution
Lanes 5-6 : Donkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97061) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3 minutes
Datasheets and documents
References
This product has been referenced in:
- Jezek J et al. Synergistic repression of thyroid hyperplasia by cyclin C and Pten. J Cell Sci 132:N/A (2019). Read more (PubMed: 31331961) »
- Jezek J et al. Mitochondrial translocation of cyclin C stimulates intrinsic apoptosis through Bax recruitment. EMBO Rep 20:e47425 (2019). Read more (PubMed: 31385392) »
