Product nameDonkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed
See all IgG secondary antibodies
Tested applicationsSuitable for: ICC/IF, IHC-P, WB, ELISA, Dot blot, IHC-Frmore details
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Rabbit IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 50% Glycerol, 0.0014% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl
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Purification notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Our Abpromise guarantee covers the use of ab7084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/400 - 1/2000.|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/1000 - 1/5000.|
|ELISA||1/4000 - 1/16000.|
|Dot blot||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
This product has been referenced in:
- Choi Y et al. Preventive effects of indole-3-carbinol against alcohol-induced liver injury in mice via antioxidant, anti-inflammatory, and anti-apoptotic mechanisms: Role of gut-liver-adipose tissue axis. J Nutr Biochem 55:12-25 (2018). Read more (PubMed: 29331880) »
- Peng EW et al. Myocardial expression of heat shock protein 70i protects early postoperative right ventricular function in cyanotic tetralogy of Fallot. J Thorac Cardiovasc Surg 141:1184-91 (2011). Read more (PubMed: 21397265) »