Key features and details
- Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (AP), pre-adsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, WB, IHC-P, ELISA
Product nameDonkey Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed
See all IgG secondary antibodies
DescriptionDonkey polyclonal Secondary Antibody to Rabbit IgG - H&L (AP), pre-adsorbed
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
Tested applicationsSuitable for: ICC, WB, IHC-P, ELISAmore details
Chicken, Cow, Goat, Human, Mouse, Pig, RatTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Other Immunogen Type corresponding to Rabbit IgG.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
Our Abpromise guarantee covers the use of ab97082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|WB||1/2500 - 1/25000. (Chemiluminescent)|
|IHC-P||1/100 - 1/1000.|
|ELISA||1/2500 - 1/25000. (Primary ELISA)|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97082 has been referenced in 2 publications.
- Rigon L et al. Targeting Brain Disease in MPSII: Preclinical Evaluation of IDS-Loaded PLGA Nanoparticles. Int J Mol Sci 20:N/A (2019). PubMed: 31022913
- Ligaba-Osena A et al. Reducing biomass recalcitrance by heterologous expression of a bacterial peroxidase in tobacco (Nicotiana benthamiana). Sci Rep 7:17104 (2017). WB . PubMed: 29213132