Product nameDonkey Anti-Rabbit IgG H&L (Biotin)
See all IgG secondary antibodies
Tested applicationsSuitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
Rabbit IgG whole molecule
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
Concentration information loading...
Purification notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Donkey IgG molecule.
Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Our Abpromise guarantee covers the use of ab6801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/3000 - 1/15000.|
|Immunomicroscopy||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
|ICC/IF||1/500 - 1/3000.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
This product has been referenced in:
- Johnson MB et al. Topical Fibronectin Improves Wound Healing of Irradiated Skin. Sci Rep 7:3876 (2017). IHC-P ; Mouse . Read more (PubMed: 28634413) »
- Ghorbani M et al. Apparent histological changes of adipocytes after treatment with CL 316,243, a ß-3-adrenergic receptor agonist. Drug Des Devel Ther 9:669-76 (2015). IHC-P . Read more (PubMed: 25709398) »