Donkey Anti-Rabbit IgG H&L (Biotin) preadsorbed (ab7082)

Submit a review Q&A (5)References (2)

Overview

  • Product name
    Donkey Anti-Rabbit IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, Immunomicroscopy, Dot blot, IHC-P, IHC-Frmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Full length native Rabbit IgG (purified).

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
ELISA
Immunomicroscopy
Dot blot
IHC-P
IHC-Fr
  • Application notes
    Dot: Use at an assay dependant dilution.
    ELISA: 1/50000 - 1/200000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Images

    • Immunohistochemistry (Frozen sections) - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin), pre-adsorbed (ab7082)
      Immunohistochemistry (Frozen sections) - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin), pre-adsorbed (ab7082)
      Ab7082 was used at dilution 1/200 with the primary antibody ab77512 in IHC-Fr. See the review on ab77512.

    References

    This product has been referenced in:
    • Chu DT  et al. C57BL/6J mice as a polygenic developmental model of diet-induced obesity. Physiol Rep 5:N/A (2017). ChIP . Read more (PubMed: 28400497) »
    • Vasistha NA  et al. Cortical and Clonal Contribution of Tbr2 Expressing Progenitors in the Developing Mouse Brain. Cereb Cortex N/A:N/A (2014). IHC (PFA fixed) . Read more (PubMed: 24927931) »
    See all 2 Publications for this product

    Publishing research using ab7082? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Question

    Hahaha, yes you are right, the original secondary was anti-rabbit, not anti-mouse.   I do appreciate the customer friendlyness you show, and I cross my fingers for getting the assay to work with the new antibodies.  

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    Answer

    I have processed your request to receive ab106843 as a replacement for ab16801. I have also sent ab97055, goat polyclonal to rat (Biotin) aswell. The ab106843 is backordered until December 12th though and so your order will ship as soon as it comes into stock. Your new order number is ******. If there is anything else I can help you with, please let me know.

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    Question

    Allright, I would like to give the ab106843 a try. Since it is a rat antibody I need another biotinylated secondary because the one we ordered previously was (of course) anti-mouse. Would you mind shipping a complimentary biotinylated anti-rat secondary ab, together with the ab106843?

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    Answer

    Thank you for your reply. I would be happy to send you ab106843 as a replacement. The secondary antibody that you originally ordered was an anti-rabbit, not an anti-mouse, is that correct? I would also be happy to send you ab97055 as well. Could you please send me the complete address that you would like these products to be shipped to in ***** Once I have that information I will go ahead and have the replacements sent to you. I look forward to your r

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    Question

      Dear John, Thanks for the quick response. Has the ab55075 any proven mouse reactivity? The web info states only human reactivity...   I don't want to spend time and reagents on trying an antibody not proven to be working on mouse GR. Please advice.  

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    Answer

    Thank you for your reply. The antibody ab55075 has not been proven to work on mouse tissue, there is a chance that the antibody will work on mouse tissue but we cannot guarantee it. The only other antibody in our catalogue that will work on mouse tissue in IHC-P, is ab106843, which is a rat monoclonal. If this antibody is not suitable then I would be happy to process a refund for you. The refund would have to go back onto the credit card that was used in the original purchase. I look forward to your response.  

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    Question

    Hi again, I have another problem now, unfortunately of a worse nature.   The anti-glutathione reductase antibody does not work for us. I've tried a number of protocol variations for IHC-P (including +/- heat antigen retrieval) without any specific staining. As a last resort we did a western on mouse tissue that we empirically know contains glutathine reductase, with various protein load and high primary antibody concentration, however without any specific bands.  

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    Answer

    I am sorry that you are having problems using ab16801 in IHC-P and in western blot. Normally I would try and offer protocol tips for the IHC-P staining but the fact that you are not getting a signal when using the antibody in western blot does show that the antibody is not working as guaranteed. I would be happy to send you a new antibody, I checked back on your original order and found the lot number of the vial of glutathione reductase that you have. Currently we have the same lot that you originally ordered for ab16801 and so I could either send you a new vial of that antibody (maybe it was affected by the transit) or I could send you a different glutathione reductase antibody, ab55075 may be a suitable alternative. If neither of these options would work for you, then I could process a refund.

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    Question

    I'm visiting Lincoln NE for collaborative research. Here I've been doing immunohistochemical experiments, using your antibodies.   When I return on Thursday I would like to bring the antibodies with me. How temperature sensitive are your antibodies? Can I put them in my hand luggage in room temperature for the 12 hours trip home, without damaging the antibodies too much? Or do I have to transport the antibodies in dry ice?   Item numbers ab22604, ab16801, ab7082.  

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    Answer

    The antibodies should be stable at room temperature for that short period of time. It at all possible though, if you can even keep them with an ice pack that would be better. If there is anything else I can help you with, please let me know.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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