Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96919)

Submit an Abreview Q&A (8)Specific References (3)

Overview

  • Product name
    Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed
  • Host species
    Donkey
  • Target species
    Rabbit
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details
  • Conjugation
    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96919)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96919)This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.
    Glial fibrillary acidic protein(GFAP) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96919) was used as a secondary antibdoy at a dilution of 1/50.
  • Immunohistochemistry (Frozen sections) - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96919)
    Immunohistochemistry (Frozen sections) - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96919)

    E14 mouse brain sample was stained with primary antibody (ab66155). The secondary antibody used was ab96919 (1:200 dilution, incubate for 1 hour at room temperature). The blue background was from DAPI.

    This image is courtesy of an anonymous Abreview

  • DyLight® - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96919)
    DyLight® - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96919)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:
  • Tschurtschenthaler M  et al. Defective ATG16L1-mediated removal of IRE1a drives Crohn's disease-like ileitis. J Exp Med 214:401-422 (2017). Read more (PubMed: 28082357) »
  • Kaylan KB  et al. Combinatorial microenvironmental regulation of liver progenitor differentiation by Notch ligands, TGFß, and extracellular matrix. Sci Rep 6:23490 (2016). Read more (PubMed: 27025873) »

See all 3 Publications for this product

Publishing research using ab96919? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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8 reviews or Q&A

10
Votes: Highest First

Buenos días ,
Me gustaría que me mandases el código para beneficiarme de la promoción de Abreviews. Tenemos decidido adquirir el anticuerpo primario del cual hemos hablado (ab5794) así como del decundario que me ...

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Tal y como hemos quedado, he creado un código de descuento: XXXXXX para testar ab5794 en células de gato mediante ICC.

El código caduca el día 20-Marzo-2013.

Este código tiene un valor de €370...

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One of your costumers intends to buy the following secondary antibodies for ICC/ IF, double staining:

1-) Donkey F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 594), preadsorbed (ab98768) - Orange/Red

2...

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Thank you for contacting us.

I think this information would need to be obtained from the manufacturer of the microscope and filters that the customer is planning touse. They would be able to exactly tell the customer which wavelengths can be...

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use of ab96919 on tissue sections

Thank you for your call.

As discussed on the phone, the ab96919 can be used in a variety of protocols and applications. Please find here also the links to our online immunohistochemistry (IHC)protocols.

IHC protocol summary: https:/...

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Product code: 96919
Inquiry: The datasheet of ab96919 shows the image of Immunohistochemistry (Frozen sections) of E14 mouse brain but for some reason IHC-Fr does not appear as a tested application. Kindly advise if the customer will get Abpromise...

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DISCOUNT CODE: 42087
Expiration date: 28/05/2012
I am very pleased to hear you would like to accept our offer and test ab96919 in IHC-Fr. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date.
...

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Inquiry: The datasheet of ab96919 shows the image of Immunohistochemistry (Frozen sections) of E14 mouse brain but for some reason IHC-Fr does not appear as a tested application. Kindly advise if the customer will get Abpromise for IHC-Fr in this case?...

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Thank you for your enquiry and your interest in our products.
I can certainly offer a discount code for testing this secondary antibody for IHC on frozen sections.
Could you please pass your customer's contact details for our internal record ...

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Dear All,



Sending you attached a list of the tendered items. You can see the products at page 3-5. Please give all of the items a check and let us know which items are best available with Abcam.



Early respo...

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Thank you for your inquiry.

We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

Please e...

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Dear Karin,

sorry, i thought i've already sent you the appendix, but there was something wrong with the address. Now here it is!

Best regards,

Thank you for providing that extra information. Out of the secondaries used, the DyLight488 (ab96919) definitely seems to be producing more diffuse, non-nuclear staining compared to the other three. It may be that this could be reduced by optimising th...

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LOT NUMBER GR63301-1 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM non-specific staining SAMPLE rat/brain PRIMARY ANTIBODY prim. AK: pCREB(rb) (Cell Signaling) 1:800 dilution: 5mg BSA in 990µl 0,05% Triton-X100 + 10µl NaN³...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that the Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody (ab96919) is not providing satisfactory results. The details provided will enable us to investigate this c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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