Donkey Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed (ab96920)

Overview

  • Product name

    Donkey Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed
    See all IgG secondary antibodies
  • Description

    Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed
  • Host species

    Donkey
  • Target species

    Rabbit
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.

     

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details
  • Conjugation

    DyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    DyLight® 550 replaces DyLight® 549.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.

DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.

ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:

  • Liubomirski Y  et al. Tumor-Stroma-Inflammation Networks Promote Pro-metastatic Chemokines and Aggressiveness Characteristics in Triple-Negative Breast Cancer. Front Immunol 10:757 (2019). Read more (PubMed: 31031757) »
  • Xiao Q  et al. DKK2 imparts tumor immunity evasion through ß-catenin-independent suppression of cytotoxic immune-cell activation. Nat Med 24:262-270 (2018). Read more (PubMed: 29431745) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your inquiry.

I strongly suggest to use a pre-absorbed secondary antibody so that the cross-binding to the two rat monoclonals can be minimized.

Since you are already using fluorescence in the green and red spectrum, I recommend to choose a color in the yellow spectrum to go with it.

These two secondaries will fit this description:

ab96920 (Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed)

Click here (or use the following: https://www.abcam.com/index.html?datasheet=96920).

ab98499 (Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed)

Click here (or use the following: https://www.abcam.com/index.html?datasheet=98499).

Please make sure that the instrument you are planning to use has the right filter sets and lasers to be able to measure all three colors together.

I wish you good luck with your experiments.

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