Donkey Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed (ab96920)
Key features and details
- Donkey Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed
- Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Overview
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Product name
Donkey Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
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Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details -
Conjugation
DyLight® 550. Ex: 562nm, Em: 576nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
DyLight® 550 replaces DyLight® 549. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/500.
DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550. |
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ICC/IF |
1/50 - 1/500.
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Flow Cyt |
1/50 - 1/200.
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Notes |
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IHC-P
1/50 - 1/500. DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (5)
ab96920 has been referenced in 5 publications.
- Decourtye-Espiard L et al. Stimulation of GHRH Neuron Axon Growth by Leptin and Impact of Nutrition during Suckling in Mice. Nutrients 15:N/A (2023). PubMed: 36904077
- Rubinstein-Achiasaf L et al. Persistent Inflammatory Stimulation Drives the Conversion of MSCs to Inflammatory CAFs That Promote Pro-Metastatic Characteristics in Breast Cancer Cells. Cancers (Basel) 13:N/A (2021). PubMed: 33806906
- Liubomirski Y et al. Tumor-Stroma-Inflammation Networks Promote Pro-metastatic Chemokines and Aggressiveness Characteristics in Triple-Negative Breast Cancer. Front Immunol 10:757 (2019). PubMed: 31031757
- Xiao Q et al. DKK2 imparts tumor immunity evasion through ß-catenin-independent suppression of cytotoxic immune-cell activation. Nat Med 24:262-270 (2018). PubMed: 29431745
- Moldavan M et al. Localization and expression of GABA transporters in the suprachiasmatic nucleus. Eur J Neurosci 42:3018-32 (2015). PubMed: 26390912