Overview

  • Product name
    Donkey Anti-Rabbit IgG H&L (DyLight® 650)
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Rabbit
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Conjugation
    DyLight® 650. Ex: 654nm, Em: 673nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    DyLight® 650 replaces DyLight® 649.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:
  • Tschurtschenthaler M  et al. Defective ATG16L1-mediated removal of IRE1a drives Crohn's disease-like ileitis. J Exp Med 214:401-422 (2017). Read more (PubMed: 28082357) »
  • Ebisawa M  et al. Significance of caveolin-1 and matrix metalloproteinase 14 gene expression in canine mammary tumours. Vet J N/A:N/A (2015). IF . Read more (PubMed: 26364240) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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