Product nameDonkey Anti-Rabbit IgG H&L (DyLight® 650)
See all IgG secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-Pmore details
ConjugationDyLight® 650. Ex: 654nm, Em: 673nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
General notesDyLight® 650 replaces DyLight® 649.
Our Abpromise guarantee covers the use of ab96894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/50 - 1/200.|
|ICC/IF||1/50 - 1/500.|
|IHC-P||1/50 - 1/500.
DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650.
This product has been referenced in:
- Wakasugi T et al. Role of smooth muscle cell p53 in pulmonary arterial hypertension. PLoS One 14:e0212889 (2019). Read more (PubMed: 30807606) »
- Gosselin EA et al. Multi-layered silk film coculture system for human corneal epithelial and stromal stem cells. J Tissue Eng Regen Med 12:285-295 (2018). Read more (PubMed: 28600807) »