Product nameDonkey anti-Rabbit IgG H&L (DyLight® 550)
See all IgG secondary antibodies
DescriptionDonkey anti-Rabbit IgG H&L (DyLight® 550)
Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-Pmore details
Chicken, Cow, Goat, Horse, Human, Mouse, Rat, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
ConjugationDyLight® 550. Ex: 562nm, Em: 576nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 550.
General notesDyLight® 550 replaces DyLight® 549.
Our Abpromise guarantee covers the use of ab98489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/50 - 1/200.|
|ICC/IF||1/50 - 1/500.|
|IHC-P||1/50 - 1/500.
DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.
This product has been referenced in:
- Wawro K et al. The role of NF-?B and Elk-1 in the regulation of mouse ADAM17 expression. Biol Open 8:N/A (2019). Read more (PubMed: 30709842) »
- Lu Y et al. Effect of midkine on gemcitabine resistance in biliary tract cancer. Int J Mol Med 41:2003-2011 (2018). Read more (PubMed: 29344648) »