Donkey Anti-Rabbit IgG H&L (FITC) (ab6798)
Key features and details
- Donkey Anti-Rabbit IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA
Related conjugates and formulations
Alexa Fluor® 405
Alexa Fluor® 488
Alexa Fluor® 488
Alexa Fluor® 555
Alexa Fluor® 568
Alexa Fluor® 594
Alexa Fluor® 647
Alexa Fluor® 680
Alexa Fluor® 750
Alexa Fluor® 790
Alkaline Phosphatase
Biotin
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 650
Gold 10nm
Gold 12nm
Gold 18nm
Gold 25nm
Gold 6nm
HRP
IRDye® 680RD
Texas Red ®
TRITC
Unconjugated
Unconjugated
Overview
-
Product name
Donkey Anti-Rabbit IgG H&L (FITC)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Tested applications
Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details -
Immunogen
Rabbit IgG whole molecule
-
Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG -
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
1/500 - 1/2500.
|
|
| ICC/IF |
1/1000 - 1/5000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| ELISA |
1/10000 - 1/50000.
|
| Notes |
|---|
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
Flow Cyt
1/500 - 1/2500. |
|
ICC/IF
1/1000 - 1/5000. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ELISA
1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (16)
ab6798 has been referenced in 16 publications.
- Bhattarai Y et al. Role of gut microbiota in regulating gastrointestinal dysfunction and motor symptoms in a mouse model of Parkinson's disease. Gut Microbes 13:1866974 (2021). PubMed: 33459114
- Moura-Assis A et al. TLR4-interactor with leucine-rich repeats (TRIL) is involved in diet-induced hypothalamic inflammation. Sci Rep 11:18015 (2021). PubMed: 34504172
- Dong Y et al. Stress-induced NLRP3 inflammasome activation negatively regulates fear memory in mice. J Neuroinflammation 17:205 (2020). PubMed: 32635937
- Müller-Komorowska D et al. Nonspecific Expression in Limited Excitatory Cell Populations in Interneuron-Targeting Cre-driver Lines Can Have Large Functional Effects. Front Neural Circuits 14:16 (2020). PubMed: 32395103
- Omidpanah S et al. Effects of Monoterpenes of Trachyspermum ammi on the Viability of Spermatogonia Stem Cells In Vitro. Plants (Basel) 9:N/A (2020). PubMed: 32182777