Donkey Anti-Rabbit IgG H&L (FITC) (ab6798)
- Datasheet
- References (11)
- Protocols
Overview
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Product nameDonkey Anti-Rabbit IgG H&L (FITC)
See all IgG secondary antibodies -
Host speciesDonkey
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Target speciesRabbit
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Tested applicationsSuitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
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Immunogen
Rabbit IgG whole molecule
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ConjugationFITC. Ex: 493nm, Em: 528nm
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
PurityAffinity purified
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Purification notesFluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG
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Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab6798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Immunomicroscopy | Use at an assay dependent concentration. | |
| Flow Cyt | 1/500 - 1/2500. | |
| ICC/IF | 1/1000 - 1/5000. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ELISA | 1/10000 - 1/50000. |
References
This product has been referenced in:
- Sun X et al. Inhibition of microRNA-155 modulates endotoxin tolerance by upregulating suppressor of cytokine signaling 1 in microglia. Exp Ther Med 15:4709-4716 (2018). Read more (PubMed: 29805490) »
- Banerjee S & Chaturvedi CM Neuroendocrine mechanism of food intake and energy regulation in Japanese quail under differential simulated photoperiodic conditions: Involvement of hypothalamic neuropeptides, AMPK, insulin and adiponectin receptors. J Photochem Photobiol B 185:10-23 (2018). IHC-P ; Quail . Read more (PubMed: 29857310) »
