Product nameDonkey Anti-Rabbit IgG H&L (FITC)
See all IgG secondary antibodies
Tested applicationsSuitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
Rabbit IgG whole molecule
ConjugationFITC. Ex: 493nm, Em: 528nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
Concentration information loading...
Purification notesFluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG
Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Our Abpromise guarantee covers the use of ab6798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Immunomicroscopy||Use at an assay dependent concentration.|
|Flow Cyt||1/500 - 1/2500.|
|ICC/IF||1/1000 - 1/5000.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||1/10000 - 1/50000.|
This product has been referenced in:
- Sun X et al. Inhibition of microRNA-155 modulates endotoxin tolerance by upregulating suppressor of cytokine signaling 1 in microglia. Exp Ther Med 15:4709-4716 (2018). Read more (PubMed: 29805490) »
- Banerjee S & Chaturvedi CM Neuroendocrine mechanism of food intake and energy regulation in Japanese quail under differential simulated photoperiodic conditions: Involvement of hypothalamic neuropeptides, AMPK, insulin and adiponectin receptors. J Photochem Photobiol B 185:10-23 (2018). IHC-P ; Quail . Read more (PubMed: 29857310) »