Donkey Anti-Rabbit IgG H&L (FITC) preadsorbed (ab7079)
Key features and details
- Donkey Anti-Rabbit IgG H&L (FITC) preadsorbed
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Donkey
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA
Overview
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Product name
Donkey Anti-Rabbit IgG H&L (FITC) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Tested applications
Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
Immunogen
Full length native Rabbit IgG (purified).
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG -
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
Clonality
Polyclonal -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | ||
Immunomicroscopy | ||
Flow Cyt | ||
IHC-P | ||
IHC-Fr | ||
ELISA |
Notes |
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IHC-P: Use at an assay dependant concentration.
IHC-Fr: Use at an assay dependant concentration.
IM: Use at an assay dependant concentration.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (3)
ab7079 has been referenced in 3 publications.
- Griner SL et al. Structure-based inhibitors of amyloid beta core suggest a common interface with tau. Elife 8:N/A (2019). PubMed: 31612856
- Singh SJ et al. Metabolic shift in density-dependent stem cell differentiation. Cell Commun Signal 15:44 (2017). PubMed: 29052507
- Guo Z et al. Antisense oligonucleotide treatment enhances the recovery of acute lung injury through IL-10-secreting M2-like macrophage-induced expansion of CD4+ regulatory T cells. J Immunol 190:4337-48 (2013). PubMed: 23514739