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    donkey-rabbit-igg-hl-fitc-preadsorbed-ab97084.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Rabbit IgG H&L (FITC) preadsorbed (ab97084)

  • Datasheet
  • SDS
Submit a review Q&A (4)References (1)

Product price, shipping and contact information

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Abpromise

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Find out more.

Key features and details

  • Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-P, Flow Cyt

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Overview

  • Product name

    Donkey Anti-Rabbit IgG H&L (FITC) preadsorbed
    See all IgG secondary antibodies
  • Description

    Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed
  • Host species

    Donkey
  • Target species

    Rabbit
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.

     

  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cytmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Human, Mouse, Pig, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Fluorophore
    • FITC

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab97084 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    1/50 - 1/500.
    IHC-P
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.
    Notes
    ICC/IF
    1/50 - 1/500.
    IHC-P
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab97084? Please let us know so that we can cite the reference in this datasheet.

    ab97084 has been referenced in 1 publication.

    • Lajko M  et al. Photoreceptor oxidative stress in hyperoxia-induced proliferative retinopathy accelerates rd8 degeneration. PLoS One 12:e0180384 (2017). IHC-P ; Mouse . PubMed: 28671996

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Question

    Thank you for the information. I will attempt to use without conjugation initially.

    Is there a protocol that you can recommend when using these antibodies and should the cells be permeabilised as the GLUT4 is translocated to the plasma membrane?

    Thanks in advance,

    Kind Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Apr 20 2012

    Answer

    We used the following protocol with Anti-Glucose Transporter GLUT4 antibody (ab62375):

    NSO cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab62375, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (https://www.abcam.com/Goat-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96899.html) at 1/500 dilution for 30 min at 22°C.

    Isotype control antibody was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This antibody also gave a positive signal in NSO cells fixed with 4% paraformaldehyde (10 min) permeabilized with 0.1% PBS-Tween used under the same conditions.

    For both of these methods permiabilisation was used.

    I hope this information has been of help. If you require any further information, please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    Answered on Apr 20 2012

    Question

    Phone call requesting information on if it would be a problem to pre-incubate the primary and secondary antibody together prior to performing FACS analysis

    Read More

    Abcam community

    Verified customer

    Asked on Apr 20 2012

    Answer

    Thank you for contacting us in regards to pre-incubating a primary and secondary together prior to performing FACS analysis.

    I have spoken to my colleague who is quite experienced in using flow cytometry and she has informed me that whilst pre-incubation can be performed, it can sometimes lead to the formation of particles which can be picked up as an event in themselves.

    If you would like to avoid using a secondary antibody altogether we have a range of conjugation kits which would allow you to conjugate your primary antibody directly. More information on these kits can be found from the following link:

    https://www.abcam.com/index.html?pageconfig=resource&rid=13148

    If contemplating this please do consult the following guide to decide if the primary antibody you wish to use is compatible:

    https://www.abcam.com/index.html?pageconfig=resource&rid=13156

    If you would like any assistance in determining this, could you please let me know the primary antibody again (unfortunately I think I took down the wrong number when speaking with you) and I can have a look into this.

    I hope this information has been of some help. If you require any further information please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    Answered on Apr 20 2012

    Question

    human tissue
    intestinal tumor as pos. crtl
    no signal at all
    4 um slides
    AR: citrate buffer (heating plate)
    block: 10% donkey serum 30min
    ab16832: 5, 10 ug/ml
    ab118519: 20 ug/ml

    suggested: try other AR, use more Ab, try HRP-secondary + hematoxyline (to check if any signal can be obtained)

    Read More

    Abcam community

    Verified customer

    Asked on Apr 19 2012

    Answer

    Thank you for contacting us.
    I am sorry to hear that you received so far no staining with the antibodies.

    As we discussed by phone, I'd suggest trying the following:

    1) try another antigen retrieval step (e.g. Tris/EDTA, pH 8)
    2)use more priamry antibody
    3) try using an HRP-conjugated secondary antibody and hematoxyline (for nuclear staining)- to check if any signal can be obtained at all, with the experimentalsetup you have.

    Right now it is difficult to determine where the problem might stem from - there could be multiple causes. Thus, please let me know what results you obtained after trying these tips and we can discuss this further.

    I wish you good luck and look forward to hear back from you.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Apr 19 2012

    Question

    Antibody did not work with A172 cells in Flow cytometry
    - No staining
    - Cell Permeabilization and fixing OK
    - Secondry antibody ab97084

    Read More

    Abcam community

    Verified customer

    Asked on Jun 22 2012

    Answer

    Thank you very much for contacting us.

    Please find attached the QC ICC/IF image for this antibody. The cell line is A172 cells. The antibodies used is at 5ug//ml and detected using Alexa Fluor 488 conjugated secondary antibody. The nuclei are stained with DAPI.

    Please note the protein location is membranous as well as cytoplasmic so in flow cytometry you will need a permabilzation step.

    I hope this information will be helpful. Should you have any further question please do not hesitate to ask.

    Read More

    Abcam Scientific Support

    Answered on Jun 22 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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