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Phone call requesting information on if it would be a problem to pre-incubate the primary and secondary antibody together prior to performing FACS analysis
Asked on Apr 20 2012
Thank you for contacting us in regards to pre-incubating a primary and secondary together prior to performing FACS analysis.
I have spoken to my colleague who is quite experienced in using flow cytometry and she has informed me that whilst pre-incubation can be performed, it can sometimes lead to the formation of particles which can be picked up as an event in themselves.
If you would like to avoid using a secondary antibody altogether we have a range of conjugation kits which would allow you to conjugate your primary antibody directly. More information on these kits can be found from the following link:
If contemplating this please do consult the following guide to decide if the primary antibody you wish to use is compatible:
If you would like any assistance in determining this, could you please let me know the primary antibody again (unfortunately I think I took down the wrong number when speaking with you) and I can have a look into this.
I hope this information has been of some help. If you require any further information please do not hesitate to contact us again.
Answered on Apr 20 2012