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Antibody did not work with A172 cells in Flow cytometry
- No staining
- Cell Permeabilization and fixing OK
- Secondry antibody ab97084
Asked on Jun 22 2012
Thank you very much for contacting us.
Please find attached the QC ICC/IF image for this antibody. The cell line is A172 cells. The antibodies used is at 5ug//ml and detected using Alexa Fluor 488 conjugated secondary antibody. The nuclei are stained with DAPI.
Please note the protein location is membranous as well as cytoplasmic so in flow cytometry you will need a permabilzation step.
I hope this information will be helpful. Should you have any further question please do not hesitate to ask.
Answered on Jun 22 2012