Product nameDonkey Anti-Rabbit IgG H&L (HRP)
See all IgG secondary antibodies
Tested applicationsSuitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
Rabbit IgG, whole molecule
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
Purification notesHorseradish Peroxidase (HRP).
Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Our Abpromise guarantee covers the use of ab6802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/3000.|
|Immunomicroscopy||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
|ICC/IF||1/500 - 1/2000.|
|IHC-P||1/500 - 1/2000.|
|IHC-Fr||1/500 - 1/2000.|
IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6802, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
This product has been referenced in:
- Staudacher I et al. Cardiovascular pharmacology of K2P17.1 (TASK-4, TALK-2) two-pore-domain K+ channels. Naunyn Schmiedebergs Arch Pharmacol N/A:N/A (2018). Read more (PubMed: 30008082) »
- He J et al. Recurrent somatic mutations ofPRKAR1Ain isolated cardiac myxoma. Oncotarget 8:103968-103974 (2017). Read more (PubMed: 29262613) »