• Product name
    Donkey Anti-Rabbit IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species
  • Target species
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Conjugation


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes
    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab97064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000.
WB 1/5000 - 1/50000. Colorimetric: Use 1/5000-1/30000, Chemiluminescent: Use 1/10000-1/50000


  • ab19968 detecting vancomycin in human serum samples by indirect ELISA. Samples were blocked with 2.5% milk for 30 minutes at 20°C and incubated with the primary antibody (1/1000 in PBS) for 1 hour at 20°C. A HRP-conjugated donkey anti-rabbit IgG H&L (ab97064) (1/1000) was used as the secondary antibody.

    See Abreview

  • Donkey polclonal secondary antibody to rabbit IgG-H&L (HRP) (ab97064) was used in a dilution of 1:5000. The first antibody was Smad4 antibody [EP618Y] (ab40759). The gel was a 4%-12% Bis-Tris Gel with MOPS Buffer. The first band is 30µg nuclear extract of a EBV-transformed B-lymphoblastoid cell line, the second band is 50µg of whole protein extract of the same EBV transformed B-LCL and the third lane is the same than in the second one but only 30µg. In this combination the secondary antibody works excellent. There is only the predicted band of 60 kDa, no addtional bands!

    This image is courtesy of Mr Daniel Meier


This product has been referenced in:
  • Lin CH & Lin CC Sitagliptin attenuates in?ammatory responses in lipopolysaccharide-stimulated cardiomyocytes via nuclear factor-?B pathway inhibition. Exp Ther Med 11:2609-2615 (2016). Read more (PubMed: 27284355) »
  • Boons E  et al. Human Exportin-1 is a Target for Combined Therapy of HIV and AIDS Related Lymphoma. EBioMedicine 2:1102-13 (2015). WB . Read more (PubMed: 26501108) »

See all 16 Publications for this product

Customer reviews and Q&As

We have not specifically tested this Anti-CD9 antibody [EPR2949] (ab92726) as a marker of urinary exosomes but I can confirm that it can be used in western blot on mouse, rat, and human samples. Additionally, I have done a Google search and found that ...

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Merci beaucoup pour votre émail et votre intérêt pour nos produits.

Nous vous avons envoyé un devis pour le ab72422 contenant aussi l'anticorps secondaire ab97064. Le ab97064 est un anticorps contre les immunoglobul...

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Thank you for contacting us.

The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not...

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Thank you for your enquiry regarding ab97064. After reviewing the WB image and the details of the experiments, it could well be that the band at around 50 kDa represents the heavy chain of the immunoglobulin on the blot. The light chain can be se...

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