Donkey Anti-Rabbit IgG H&L (HRP) (ab97064)
Key features and details
- Donkey Anti-Rabbit IgG H&L (HRP)
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Donkey Anti-Rabbit IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
Conjugation notes
Molar enzyme/ antibody protein ratio is 4:1 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent dilution.
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| IHC-P |
1/200 - 1/5000.
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| ELISA |
1/10000 - 1/100000.
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| WB | (1) |
1/5000 - 1/50000. Colorimetric: Use 1/5000-1/30000, Chemiluminescent: Use 1/10000-1/50000
|
| Notes |
|---|
|
ICC
Use at an assay dependent dilution. |
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IHC-P
1/200 - 1/5000. |
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ELISA
1/10000 - 1/100000. |
|
WB
1/5000 - 1/50000. Colorimetric: Use 1/5000-1/30000, Chemiluminescent: Use 1/10000-1/50000 |
Images
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ELISA - Donkey Anti-Rabbit IgG H&L (HRP) (ab97064)This image is courtesy of an Abreview submitted by Julie Richards.
ab19968 detecting vancomycin in human serum samples by indirect ELISA. Samples were blocked with 2.5% milk for 30 minutes at 20°C and incubated with the primary antibody (1/1000 in PBS) for 1 hour at 20°C. A HRP-conjugated donkey anti-rabbit IgG H&L (ab97064) (1/1000) was used as the secondary antibody.
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Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab97064)Donkey polclonal secondary antibody to rabbit IgG-H&L (HRP) (ab97064) was used in a dilution of 1:5000. The first antibody was Smad4 antibody [EP618Y] (ab40759). The gel was a 4%-12% Bis-Tris Gel with MOPS Buffer. The first band is 30µg nuclear extract of a EBV-transformed B-lymphoblastoid cell line, the second band is 50µg of whole protein extract of the same EBV transformed B-LCL and the third lane is the same than in the second one but only 30µg. In this combination the secondary antibody works excellent. There is only the predicted band of 60 kDa, no addtional bands!
This image is courtesy of Mr Daniel Meier
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (27)
ab97064 has been referenced in 27 publications.
- Leader Y et al. The upstream 5' splice site remains associated to the transcription machinery during intron synthesis. Nat Commun 12:4545 (2021). PubMed: 34315864
- Zhuravlev AV et al. 3-Hydroxykynurenine in Regulation of Drosophila Behavior: The Novel Mechanisms for Cardinal Phenotype Manifestations. Front Physiol 11:971 (2020). PubMed: 32848886
- Kurokawa K et al. Leukemia Inhibitory Factor Participates in the Formation of Stress Adaptation via Hippocampal Myelination in Mice. Neuroscience 446:1-13 (2020). PubMed: 32866601
- Harun-Or-Rashid M & Hallböök F Alpha 2-Adrenergic Receptor Agonist Brimonidine Stimulates ERK1/2 and AKT Signaling via Transactivation of EGF Receptors in the Human MIO-M1 Müller Cell Line. Curr Eye Res 44:34-45 (2019). PubMed: 30198788
- Glaich O et al. DNA methylation directs microRNA biogenesis in mammalian cells. Nat Commun 10:5657 (2019). PubMed: 31827083