Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7083)

Submit an Abreview Q&A (3)Specific References (3)

Overview

  • Product name
    Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Full length native Rabbit IgG (purified).

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/3000.
IHC-P Use at an assay dependent dilution.
WB 1/1000 - 1/3000.
ELISA 1/7500 - 1/30000.
Immunomicroscopy Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

References

This product has been referenced in:
  • Abdulsid A  et al. Heat shock protein 70 expression is spatially distributed in human placenta and selectively upregulated during labor and preeclampsia. PLoS One 8:e54540 (2013). WB . Read more (PubMed: 23382911) »
  • Faulkner S  et al. A comparison of the bovine uterine and plasma proteome using iTRAQ proteomics. Proteomics 12:2014-23 (2012). Read more (PubMed: 22623423) »

See all 3 Publications for this product

Publishing research using ab7083? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

10
Votes: Highest First

Again, thank you so much for the insight. I did the experiment using both adult rat hippocampus and 3-week-old pup liver against B-actin as control. Apparently, both proteins showed a weak bands, the former was expressed at around ˜35kDa and the latter...

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Thank you for contacting us again and sending this new information.

The WB image looks very convincing and proofs that the antibody is actually picking up its target as it should.

Regarding the double band in the pup sample it may be ...

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I truly appreciate your immediate reply especially at this time. From your suggestion, I will try to do a positive control run using a female adult hippocampus sample. Will that be okay? Regarding my inquiry, could you enlighten me on the expression at...

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Thank you for your reply.

Unfortunately, it is quite hard to get some information if this protein expressed in the rat hippocampus. However, I checked the SwissProt site for more information regardingthis for the human protein, and it looks li...

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I recently ordered Anti-Folate binding protein primary antibody (EPR4708) Cat # ab125030 and donkey anti-rabbit IgG H&L (HRP) secondary anitbody Cat # ab7083 to measure the expression of FBP1 (Folate receptor alpha) that has a predicted band ...

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Thank you for resending the image. Based on your description of the upper band as being ˜ 25kD it seems this could be the correct band, as the size can vary a few kD just based on the gel, buffer, etc. Adding a negative control could help ensure that t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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