Overview

  • Product name
    Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Full length native Rabbit IgG (purified).

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads
  • Conjugation notes
    Horseradish Peroxidase (HRP)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/3000.
IHC-P Use at an assay dependent dilution.
WB 1/1000 - 1/3000.
ELISA 1/7500 - 1/30000.
Immunomicroscopy Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

References

This product has been referenced in:
  • Yang H  et al. Emulsified isoflurane treatment inhibits the cell cycle and respiration of human bronchial epithelial 16HBE cells in a p53-independent manner. Mol Med Rep 14:349-54 (2016). Read more (PubMed: 27176636) »
  • Abdulsid A  et al. Heat shock protein 70 expression is spatially distributed in human placenta and selectively upregulated during labor and preeclampsia. PLoS One 8:e54540 (2013). WB . Read more (PubMed: 23382911) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us again and sending this new information.

The WB image looks very convincing and proofs that the antibody is actually picking up its target as it should.

Regarding the double band in the pup sample it may be that the antibody is indeed picking up the non-glycosylated form. In addition, the double band may be due to a low glycosylation level or may be the protein chain plus the C-terminal pro-peptide (approx. 27 kDa).

I hope this information is again helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your reply.

Unfortunately, it is quite hard to get some information if this protein expressed in the rat hippocampus. However, I checked the SwissProt site for more information regardingthis for the human protein, and it looks like in human it is only expressed in the cerebellum. I would therefore suggest rather to use a whole brain or cerebellum lysate in case the expression pattern is the same as in humans. Kidneys do also show a high expression level in rats according to UniGene.

To be honest, I cannot tell you if the signal at 10 kDa is specific or not. From checking the SwissProt side, I would say it is probably non-specific, but as I cannot find any information there for the rat protein, it is rather a guess than really knowing. In addition, UniGene reports anon-existing expression level in liver.

Sorry to disappoint here that I cannot help to solve the riddle at the moment.

Please let me know if the new samples provide the expected results.

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Answer

Thank you for resending the image. Based on your description of the upper band as being ˜ 25kD it seems this could be the correct band, as the size can vary a few kD just based on the gel, buffer, etc. Adding a negative control could help ensure that this is not a non-specific band. Please let me know if you have any further questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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