Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7083)
Key features and details
- Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Fr
Overview
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Product name
Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
Immunogen
Full length native Rabbit IgG (purified).
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads -
Conjugation notes
Horseradish Peroxidase (HRP) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000 - 1/3000.
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IHC-P |
Use at an assay dependent dilution.
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|
WB |
1/1000 - 1/3000.
|
|
ELISA |
1/7500 - 1/30000.
|
|
Immunomicroscopy |
Use at an assay dependent dilution.
|
|
Dot blot |
Use at an assay dependent dilution.
|
|
IHC-Fr |
Use at an assay dependent dilution.
|
Notes |
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ICC/IF
1/1000 - 1/3000. |
IHC-P
Use at an assay dependent dilution. |
WB
1/1000 - 1/3000. |
ELISA
1/7500 - 1/30000. |
Immunomicroscopy
Use at an assay dependent dilution. |
Dot blot
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab7083 has been referenced in 8 publications.
- Liu CY et al. Copine-7 is required for REM sleep regulation following cage change or water immersion and restraint stress in mice. Neurosci Res N/A:N/A (2020). PubMed: 32283105
- Pichitpunpong C et al. Phenotypic subgrouping and multi-omics analyses reveal reduced diazepam-binding inhibitor (DBI) protein levels in autism spectrum disorder with severe language impairment. PLoS One 14:e0214198 (2019). PubMed: 30921354
- Fan J et al. Hydrogen sulfide lowers hyperhomocysteinemia dependent on cystathionine ? lyase S-sulfhydration in ApoE-knockout atherosclerotic mice. Br J Pharmacol 176:3180-3192 (2019). PubMed: 31140595
- Chen J et al. Long non-coding RNA-HOTAIR promotes the progression of sepsis by acting as a sponge of miR-211 to induce IL-6R expression. Exp Ther Med 18:3959-3967 (2019). PubMed: 31656541
- Yang H et al. Emulsified isoflurane treatment inhibits the cell cycle and respiration of human bronchial epithelial 16HBE cells in a p53-independent manner. Mol Med Rep 14:349-54 (2016). PubMed: 27176636
- Abdulsid A et al. Heat shock protein 70 expression is spatially distributed in human placenta and selectively upregulated during labor and preeclampsia. PLoS One 8:e54540 (2013). WB . PubMed: 23382911
- Faulkner S et al. A comparison of the bovine uterine and plasma proteome using iTRAQ proteomics. Proteomics 12:2014-23 (2012). PubMed: 22623423
- Kashino SS et al. Initiation of acquired immunity in the lungs of mice lacking lymph nodes after infection with aerosolized Mycobacterium tuberculosis. Am J Pathol 176:198-204 (2010). IHC-P . PubMed: 20008132