Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7083)
Key features and details
- Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Fr
Overview
-
Product name
Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
Immunogen
Full length native Rabbit IgG (purified).
-
Conjugation
HRP
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
-
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads -
Conjugation notes
Horseradish Peroxidase (HRP) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/1000 - 1/3000.
|
|
IHC-P |
Use at an assay dependent dilution.
|
|
WB |
1/1000 - 1/3000.
|
|
ELISA |
1/7500 - 1/30000.
|
|
Immunomicroscopy |
Use at an assay dependent dilution.
|
|
Dot blot |
Use at an assay dependent dilution.
|
|
IHC-Fr |
Use at an assay dependent dilution.
|
Notes |
---|
ICC/IF
1/1000 - 1/3000. |
IHC-P
Use at an assay dependent dilution. |
WB
1/1000 - 1/3000. |
ELISA
1/7500 - 1/30000. |
Immunomicroscopy
Use at an assay dependent dilution. |
Dot blot
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (18)
ab7083 has been referenced in 18 publications.
- Thongkorn S et al. Investigation of autism-related transcription factors underlying sex differences in the effects of bisphenol A on transcriptome profiles and synaptogenesis in the offspring hippocampus. Biol Sex Differ 14:8 (2023). PubMed: 36803626
- Lertpanprom M et al. High expression of protein tyrosine phosphatase receptor S (PTPRS) is an independent prognostic marker for cholangiocarcinoma. Front Public Health 10:835914 (2022). PubMed: 35991009
- Jiang S et al. Hydrogen sulphide reduces hyperhomocysteinaemia-induced endothelial ER stress by sulfhydrating protein disulphide isomerase to attenuate atherosclerosis. J Cell Mol Med 25:3437-3448 (2021). PubMed: 33675119
- Cui X et al. MiR-302b-5p enhances the neuroprotective effect of IGF-1 in methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson's disease by regulating inducible nitric-oxide synthase. Cell Biochem Funct N/A:N/A (2020). PubMed: 32474958
- Liu CY et al. Copine-7 is required for REM sleep regulation following cage change or water immersion and restraint stress in mice. Neurosci Res N/A:N/A (2020). PubMed: 32283105