Overview

  • Product name

    Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Description

    Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed
  • Host species

    Donkey
  • Target species

    Rabbit
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.

     

  • Tested applications

    Suitable for: ICC, IHC-P, WB, ELISAmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Human, Mouse, Pig, Rat more details
  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
WB 1/5000 - 1/50000. (Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000)
ELISA 1/10000 - 1/100000. (Primary ELISA)

Images

  • ab134047 staining VCAM1 in mouse aorta tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol and blocked with 10% normal donkey serum for 2 hours at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 0.1% Triton + 2% normal donkey serum) for 12 hours at 4°C. A HRP-conjugated donkey anti-rabbit IgG H&L (ab97085) (1/1000) was used as the secondary antibody

    See Abreview

References

This product has been referenced in:

  • Chalenko Y  et al. Hepatoprotective Activity of InlB321/15, the HGFR Ligand of Bacterial Origin, in CCI4-Induced Acute Liver Injury Mice. Biomedicines 7:N/A (2019). Read more (PubMed: 30979058) »
  • Perez Y  et al. Mutations in the microtubule-associated protein MAP11 (C7orf43) cause microcephaly in humans and zebrafish. Brain 142:574-585 (2019). Read more (PubMed: 30715179) »
See all 6 Publications for this product

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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