Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97085)

Submit an Abreview Q&A (1)Specific References (2)

Overview

  • Product name
    Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed
  • Host species
    Donkey
  • Target species
    Rabbit
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
  • Tested applications
    Suitable for: ICC, IHC-P, WB, ELISAmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Human, Mouse, Pig, Rat more details
  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
WB 1/5000 - 1/50000. (Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000)
ELISA 1/10000 - 1/100000. (Primary ELISA)

Images

  • Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97085)
    Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97085)This image is courtesy of an Abreview submitted by Anna Maksin.

    ab134047 staining VCAM1 in mouse aorta tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol and blocked with 10% normal donkey serum for 2 hours at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 0.1% Triton + 2% normal donkey serum) for 12 hours at 4°C. A HRP-conjugated donkey anti-rabbit IgG H&L (ab97085) (1/1000) was used as the secondary antibody

    See Abreview

References

This product has been referenced in:
  • Grois L  et al. Combined Inhibition of the Renin-Angiotensin System and Neprilysin Positively Influences Complex Mitochondrial Adaptations in Progressive Experimental Heart Failure. PLoS One 12:e0169743 (2017). Read more (PubMed: 28076404) »
  • Kadir R  et al. ALFY-Controlled DVL3 Autophagy Regulates Wnt Signaling, Determining Human Brain Size. PLoS Genet 12:e1005919 (2016). Read more (PubMed: 27008544) »

See all 2 Publications for this product

Publishing research using ab97085? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

10
Votes: Highest First

Dear All,

Please send us C.O.A for items:

1. ab86665, Rabbit polyclonal to Ornithine Carbamoyltransferase, 100 µg

P.O: 89200

Inv: 1343565

Date: August 03 2012

Lot: GR2107

Read More

Thank you for your enquiry.

I am pleased to provide the CoC for these antibodies.

If you have any further questions, please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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