Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97085)
Key features and details
- Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC, IHC-P, WB, ELISA
Overview
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Product name
Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed
See all IgG secondary antibodies -
Description
Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed -
Host species
Donkey -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
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Tested applications
Suitable for: ICC, IHC-P, WB, ELISAmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Human, Mouse, Pig, Rat more details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
Our Abpromise guarantee covers the use of ab97085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use at an assay dependent dilution. | |
| IHC-P | 1/200 - 1/5000. | |
| WB | 1/5000 - 1/50000. (Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000) | |
| ELISA | 1/10000 - 1/100000. (Primary ELISA) |
Images
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Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97085)This image is courtesy of an Abreview submitted by Anna Maksin.ab134047 staining VCAM1 in mouse aorta tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol and blocked with 10% normal donkey serum for 2 hours at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 0.1% Triton + 2% normal donkey serum) for 12 hours at 4°C. A HRP-conjugated donkey anti-rabbit IgG H&L (ab97085) (1/1000) was used as the secondary antibody
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (9)
ab97085 has been referenced in 9 publications.
- Chalenko Y et al. Hepatoprotective Activity of InlB321/15, the HGFR Ligand of Bacterial Origin, in CCI4-Induced Acute Liver Injury Mice. Biomedicines 7:N/A (2019). PubMed: 30979058
- Perez Y et al. Mutations in the microtubule-associated protein MAP11 (C7orf43) cause microcephaly in humans and zebrafish. Brain 142:574-585 (2019). PubMed: 30715179
- Jdey W et al. AsiDNA Treatment Induces Cumulative Antitumor Efficacy with a Low Probability of Acquired Resistance. Neoplasia 21:863-871 (2019). PubMed: 31362243
- Chalenko Y et al. Phylogenetically Defined Isoforms of Listeria monocytogenes Invasion Factor InlB Differently Activate Intracellular Signaling Pathways and Interact with the Receptor gC1q-R. Int J Mol Sci 20:N/A (2019). PubMed: 31450632
- Li L et al. Valsartan inhibits RhoA-ROCK2-MYL pathway in rat model of alcoholic cardiomyopathy. Exp Ther Med 18:4313-4321 (2019). PubMed: 31777538
- Siegenthaler B et al. The Bromodomain Inhibitor N-Methyl pyrrolidone Prevents Osteoporosis and BMP-Triggered Sclerostin Expression in Osteocytes. Int J Mol Sci 19:N/A (2018). PubMed: 30366476
- Grois L et al. Combined Inhibition of the Renin-Angiotensin System and Neprilysin Positively Influences Complex Mitochondrial Adaptations in Progressive Experimental Heart Failure. PLoS One 12:e0169743 (2017). PubMed: 28076404
- Ishikawa H et al. Stability of serum high-density lipoprotein-microRNAs for preanalytical conditions. Ann Clin Biochem 54:134-142 (2017). PubMed: 27166305
- Kadir R et al. ALFY-Controlled DVL3 Autophagy Regulates Wnt Signaling, Determining Human Brain Size. PLoS Genet 12:e1005919 (2016). PubMed: 27008544
