Product nameDonkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
See all IgG secondary antibodies
DescriptionDonkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
SpecificityThe antibody reacts with the heavy chains on rabbit IgG and with the light chains common to most rabbit immuno- globulins.
Tested applicationsSuitable for: WB, In-Cell ELISAmore details
Chicken, Cow, Goat, Guinea pig, Horse, Human, Mouse, Rat, Sheep, Syrian hamsterTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Full length protein corresponding to Rabbit IgG. Whole molecule.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.01% Sodium azide
Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer.
Concentration information loading...
Purification notesAffinity chromatography purified using antigens coupled to agarose beads.
Our Abpromise guarantee covers the use of ab216779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/25000.|
|In-Cell ELISA||1/200 - 1/800.|
Lanes 1-3 : Anti-GAPDH antibody [6C5] - Loading Control (ab8245) at 1/2000 dilution (Anti-GAPDH antibody [6C5])
Lane 4 : Anti-GAPDH antibody [6C5] - Loading Control (ab8245) at 1/2000 dilution
Lane 1 : Wild-type HAP1 cell lysate (20 µg)
Lane 2 : NF-?B p60 knockout HAP1 cell lysate (20 µg)
Lane 3 : HeLa cell lysate (20 µg)
Lane 4 : A431 cell lysate (20 µg)
Lane 1 : Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779) at 1/10000 dilution (Donkey Anti-Rabbit IgG H&L (IRDye® 680RD)- ab216779)
Lanes 2-4 : Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779) at 1/10000 dilution (Donkey Anti-Rabbit IgG H&L (IRDye® 680RD))
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab140751 overnight at 4°C. Antibody binding was detected using the Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216779 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Excitation and emission spectra of IRDye® 680RD Ex: 680, Em:694.
ab216779 has not yet been referenced specifically in any publications.