Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779)

Overview

  • Product name

    Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
    See all IgG secondary antibodies
  • Description

    Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
  • Host species

    Donkey
  • Target species

    Rabbit
  • Specificity

    The antibody reacts with the heavy chains on rabbit IgG and with the light chains common to most rabbit immuno- globulins.
  • Tested applications

    Suitable for: WB, In-Cell ELISAmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Horse, Human, Mouse, Rat, Sheep, Syrian hamster more details
  • Immunogen

    Full length protein corresponding to Rabbit IgG. Whole molecule.

  • Conjugation

    IRDye® 680RD

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.01% Sodium azide
    Constituent: PBS

    Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer.
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Affinity chromatography purified using antigens coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab216779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/25000.
In-Cell ELISA 1/200 - 1/800.

Images

  • Lanes 1-3 : Anti-GAPDH antibody [6C5] - Loading Control (ab8245) at 1/2000 dilution (Anti-GAPDH antibody [6C5])
    Lane 4 : Anti-GAPDH antibody [6C5] - Loading Control (ab8245) at 1/2000 dilution

    Lane 1 : Wild-type HAP1 cell lysate (20 µg)
    Lane 2 : NF-?B p60 knockout HAP1 cell lysate (20 µg)
    Lane 3 : HeLa cell lysate (20 µg)
    Lane 4 : A431 cell lysate (20 µg)

    Secondary
    Lane 1 : Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779) at 1/10000 dilution (Donkey Anti-Rabbit IgG H&L (IRDye® 680RD)- ab216779)
    Lanes 2-4 : Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779) at 1/10000 dilution (Donkey Anti-Rabbit IgG H&L (IRDye® 680RD))


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab140751 overnight at 4°C. Antibody binding was detected using the Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216779 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

  • Excitation and emission spectra of IRDye® 680RD Ex: 680, Em:694.

References

ab216779 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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