Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed (ab7080)
Key features and details
- Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed
- Conjugation: TRITC. Ex: 547nm, Em: 572nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA
Related conjugates and formulations
Alexa Fluor® 405
Alexa Fluor® 488
Alexa Fluor® 555
Alexa Fluor® 594
Alexa Fluor® 647
Alexa Fluor® 680
Alexa Fluor® 750
Alexa Fluor® 790
Alkaline Phosphatase
Biotin
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 650
FITC
Gold 10nm
Gold 12nm
Gold 18nm
Gold 25nm
Gold 6nm
HRP
IRDye® 680RD
Texas Red ®
Unconjugated
Unconjugated
Unconjugated
Unconjugated
Overview
-
Product name
Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Tested applications
Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
Immunogen
Full length native Rabbit IgG (purified).
-
Conjugation
TRITC. Ex: 547nm, Em: 572nm
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG -
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| Immunomicroscopy | ||
| Flow Cyt | ||
| IHC-P | ||
| IHC-Fr | ||
| ELISA |
| Notes |
|---|
Application notes
Flow Cyt: Use at an assay dependant concentration.
IHC-P: Use at an assay dependant concentration.
IHC-Fr: Use at an assay dependant concentration.
IM: Use at an assay dependant concentration.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependant concentration.
IHC-Fr: Use at an assay dependant concentration.
IM: Use at an assay dependant concentration.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (1)
ab7080 has been referenced in 1 publication.
- Zhang J et al. Dlgap1 negatively regulates browning of white fat cells through effects on cell proliferation and apoptosis. Lipids Health Dis 19:39 (2020). PubMed: 32169116
