Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed (ab7080)

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Overview

  • Product name

    Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed
    See all IgG secondary antibodies

  • Host species

    Donkey

  • Target species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details

  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details

  • Immunogen

    Full length native Rabbit IgG (purified).

  • Conjugation

    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG

  • Conjugation notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Immunomicroscopy
Flow Cyt
IHC-P
IHC-Fr
ELISA
  • Application notes
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Images

    • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed (ab7080)
      Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (TRITC) preadsorbed (ab7080)
      Ab7080 was used at dilution 1/200 with the primary antibody ab2900 in ICC/IF. See the review on ab2900.

    References

    ab7080 has not yet been referenced specifically in any publications.

    Publishing research using ab7080? Please let us know so that we can cite the reference in this datasheet.

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