Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150154)

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Overview

  • Product name
    Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 555), pre-adsorbed
  • Host species
    Donkey
  • Target species
    Rat
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • Minimal
    cross-reactivity

    Human, Mouse more details
  • Immunogen

    Synthetic peptide corresponding to IgG.

  • Conjugation
    Alexa Fluor® 555. Ex: 555nm, Em: 565nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
ELISA Use at an assay dependent concentration. Use at an assay dependent dilution
IHC-P Use at an assay dependent concentration. Use at an assay dependent dilution
Flow Cyt 1/2000.

Images

  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150154)
    Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150154)

    ICC/IF image of ab6160 in HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2µg/ml) overnight at +4°C. The secondary antibody ab150154 (shown in yellow) was used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150154)
    Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150154)

References

This product has been referenced in:
  • Yui S  et al. YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration. Cell Stem Cell 22:35-49.e7 (2018). Read more (PubMed: 29249464) »

See 1 Publication for this product

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Histone H3 phospho S28 staining human iPSC derived neurons by ICC/IF

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Abreviews
Application
Immunocytochemistry/ Immunofluorescence
iPS derived neurons were fixed in 4% formaldehyde (10 min) and then incubated in 1% serum, 0.1% Triton, 0.1% BSA in PBS for 30 mins to permeabilise the cells and block non-specific binding. The cells were then stained with antibody ab10543 (Histone H3 phospho S28 at 1/300 dilution) for 1h at room temp. The secondary antibody was Donkey Anti-Rat IgG H&L Alexa 55 (ab150154) (red) at 1:500 dilution and incubated for 1h at room temp. Total cells were stained with DAPI (blue). Excellent positive Histone H3 phospho S28 staining was observed in the nuclei of cycling cells (red).
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Submitted Jan 06 2015

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