Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150154)
Key features and details
- Donkey polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 555), pre-adsorbed
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt
Overview
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Product name
Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed
See all IgG secondary antibodies -
Description
Donkey polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 555), pre-adsorbed -
Host species
Donkey -
Target species
Rat -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. -
Tested applications
Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details -
Minimal
cross-reactivity
Human, Mouse more details -
Immunogen
Synthetic peptide corresponding to IgG.
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Conjugation
Alexa Fluor® 555. Ex: 555nm, Em: 565nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Associated products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
1/200 - 1/1000.
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| ELISA |
Use at an assay dependent concentration. Use at an assay dependent dilution
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| IHC-P |
Use at an assay dependent concentration. Use at an assay dependent dilution
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| Flow Cyt |
1/2000.
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
1/200 - 1/1000. |
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ELISA
Use at an assay dependent concentration. Use at an assay dependent dilution |
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IHC-P
Use at an assay dependent concentration. Use at an assay dependent dilution |
|
Flow Cyt
1/2000. |
Images
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150154)
ICC/IF image of ab6160 in HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 2µg/ml) overnight at +4°C. The secondary antibody ab150154 (shown in yellow) was used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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Alexa Fluor® - Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150154)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab150154 has been referenced in 9 publications.
- Wang Z et al. Using apelin-based synthetic Notch receptors to detect angiogenesis and treat solid tumors. Nat Commun 11:2163 (2020). PubMed: 32358530
- Wang Z et al. Mfsd2a and Spns2 are essential for sphingosine-1-phosphate transport in the formation and maintenance of the blood-brain barrier. Sci Adv 6:eaay8627 (2020). PubMed: 32523984
- Tan W et al. Improvement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based Proteomics. Oxid Med Cell Longev 2020:8683404 (2020). PubMed: 32566106
- Hoshino Y et al. The adeno-associated virus rh10 vector is an effective gene transfer system for chronic spinal cord injury. Sci Rep 9:9844 (2019). PubMed: 31285460
- Saaber F et al. ACKR3 Regulation of Neuronal Migration Requires ACKR3 Phosphorylation, but Not ß-Arrestin. Cell Rep 26:1473-1488.e9 (2019). PubMed: 30726732
- Steevens AR et al. SOX2 is required for inner ear growth and cochlear nonsensory formation before sensory development. Development 146:N/A (2019). PubMed: 31152002
- Kobayashi Y & Segi-Nishida E Search for factors contributing to resistance to the electroconvulsive seizure treatment model using adrenocorticotrophic hormone-treated mice. Pharmacol Biochem Behav 186:172767 (2019). PubMed: 31491434
- Nascimento MA et al. Fractone Bulbs Derive from Ependymal Cells and Their Laminin Composition Influence the Stem Cell Niche in the Subventricular Zone. J Neurosci 38:3880-3889 (2018). PubMed: 29530987
- Yui S et al. YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration. Cell Stem Cell 22:35-49.e7 (2018). PubMed: 29249464