Donkey Anti-Rat IgG H&L (Alexa Fluor® 680) preadsorbed (ab175777)
Key features and details
- Donkey Anti-Rat IgG H&L (Alexa Fluor® 680) preadsorbed
- Conjugation: Alexa Fluor® 680. Ex: 679nm, Em: 702nm
- Host species: Donkey
- Suitable for: WB
Related conjugates and formulations
Overview
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Product name
Donkey Anti-Rat IgG H&L (Alexa Fluor® 680) preadsorbed
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rat -
Tested applications
Suitable for: WBmore details -
Minimal
cross-reactivity
Human, Mouse more details -
Immunogen
The details of the immunogen for this antibody are not available.
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Conjugation
Alexa Fluor® 680. Ex: 679nm, Em: 702nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
General notes
We batch test Donkey Anti-Rat IgG H&L (Alexa Fluor® 680) preadsorbed, ab175777 in fluorescent WB. Although we don’t batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Donkey Anti-Rat IgG H&L (Alexa Fluor® 680) preadsorbed, ab175777 in these applications.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000.
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| Notes |
|---|
|
WB
1/10000. |
Images
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Western blot - Donkey Anti-Rat IgG H&L (Alexa Fluor® 680) preadsorbed (ab175777)All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 5 µg
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 2 µg
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 1 µg
Secondary
All lanes : Donkey Anti-Rat IgG H&L (Alexa Fluor® 680) preadsorbed (ab175777) at 1/10000 dilution
Observed band size: 52 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6160 overnight at 4°C. Antibody binding was detected using ab175777 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab175777 has not yet been referenced specifically in any publications.