Donkey anti-Rat IgG H&L (PE) (ab7008)
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Overview
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Product nameDonkey anti-Rat IgG H&L (PE)
See all IgG secondary antibodies -
DescriptionDonkey anti-Rat IgG H&L (PE)
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Host speciesDonkey
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Target speciesRat
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Tested applicationsSuitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Frmore details
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Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details -
Immunogen
Rat IgG whole molecule
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ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPrepared from monospecific antiserum by immunoaffinity chromatography using rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
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Conjugation notesR-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
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ClonalityPolyclonal
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General notes
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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Research areas
Applications
Our Abpromise guarantee covers the use of ab7008 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| Immunomicroscopy | 1/50 - 1/200. | |
| Flow Cyt | 1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. |
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| IHC-P | 1/50 - 1/200. | |
| IHC-Fr | 1/50 - 1/200. |
Images
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Immunocytochemistry/ Immunofluorescence - Donkey anti-Rat IgG H&L (PE) secondary antibody (ab7008)This image is courtesy of an anonymous AbreviewImmunocytochemsity/Immunofluorescence analysis of lung tissue using Donkey anti-rabbit IgG H&L (PE) secondary antibody (ab7008).
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Flow Cytometry - Donkey F(ab')2 polyclonal Secondary Antibody to Rat IgG - H&L (PE), pre-adsorbed (ab7008)
Datasheets and documents
References
ab7008 has not yet been referenced specifically in any publications.
