Donkey anti-Rat IgG H&L (PE) (ab7008)

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Overview

  • Product name
    Donkey anti-Rat IgG H&L (PE)
    See all IgG secondary antibodies
  • Description
    Donkey anti-Rat IgG H&L (PE)
  • Host species
    Donkey
  • Target species
    Rat
  • Tested applications
    Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Frmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details
  • Immunogen

    Rat IgG whole molecule

  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Prepared from monospecific antiserum by immunoaffinity chromatography using rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • Conjugation notes
    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • Clonality
    Polyclonal
  • General notes


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Immunomicroscopy 1/50 - 1/200.
Flow Cyt 1/100 - 1/250.
The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
IHC-P 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.

Images

  • Immunocytochemistry/ Immunofluorescence - Donkey anti-Rat IgG H&L (PE) secondary antibody (ab7008)
    Immunocytochemistry/ Immunofluorescence - Donkey anti-Rat IgG H&L (PE) secondary antibody (ab7008)This image is courtesy of an anonymous Abreview

    Immunocytochemsity/Immunofluorescence analysis of lung tissue using Donkey anti-rabbit IgG H&L (PE) secondary antibody (ab7008).

  • Flow Cytometry - Donkey F(ab')2 polyclonal Secondary Antibody to Rat IgG - H&L (PE), pre-adsorbed (ab7008)
    Flow Cytometry - Donkey F(ab')2 polyclonal Secondary Antibody to Rat IgG - H&L (PE), pre-adsorbed (ab7008)
    Ab7008 was used with the primary antibody ab19765 in Flow Cyt. See Abreview on ab19765.

References

ab7008 has not yet been referenced specifically in any publications.

Publishing research using ab7008? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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lung tissue IF stained with ab7008

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
IF stain in lung tissue and using secondary antibody.
this antibody is good because it is very specific and has good color.

Abcam user community

Verified customer

Submitted Dec 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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