Donkey anti-Rat IgG H&L (PE) (ab7008)
Key features and details
- Donkey anti-Rat IgG H&L (PE)
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Donkey
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr
Related conjugates and formulations
Overview
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Product name
Donkey anti-Rat IgG H&L (PE)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rat -
Tested applications
Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Frmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details -
Immunogen
Rat IgG whole molecule
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Conjugation
PE. Ex: 488nm, Em: 575nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Prepared from monospecific antiserum by immunoaffinity chromatography using rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation -
Conjugation notes
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 -
Clonality
Polyclonal -
General notes
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7008 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
1/100 - 1/250.
|
| Immunomicroscopy |
1/50 - 1/200.
|
|
| Flow Cyt |
1/100 - 1/250.
The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. |
|
| IHC-P |
1/50 - 1/200.
|
|
| IHC-Fr |
1/50 - 1/200.
|
| Notes |
|---|
|
ICC/IF
1/100 - 1/250. |
|
Immunomicroscopy
1/50 - 1/200. |
|
Flow Cyt
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. |
|
IHC-P
1/50 - 1/200. |
|
IHC-Fr
1/50 - 1/200. |
Images
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Immunocytochemistry/ Immunofluorescence - Donkey anti-Rat IgG H&L (PE) (ab7008)This image is courtesy of an anonymous Abreview
Immunocytochemsity/Immunofluorescence analysis of lung tissue using Donkey anti-rabbit IgG H&L (PE) secondary antibody (ab7008).
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Flow Cytometry - Donkey anti-Rat IgG H&L (PE) (ab7008)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab7008 has not yet been referenced specifically in any publications.