Overview

  • Product name

    Donkey Anti-Sheep IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Sheep
  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, IHC-P, WB, ELISA, Immunomicroscopy, Dot blotmore details
  • Immunogen

    Full length native Sheep IgG (purified).

  • Conjugation

    Alkaline Phosphatase

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 8.00
    Preservative: 0.01% Sodium azide
    Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Conjugation notes

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality

    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-Fr
IHC-P
WB
ELISA
Immunomicroscopy
Dot blot
  • Application notes
    Dot: Use at an assay dependant dilution.
    ELISA: 1/3000 - 1/14000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    Question
    Answer

    This antibody is affinity purified by Sheep IgG conjugated agarose bead, not adsorped by other serum protein.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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