Overview

  • Product name

    Donkey Anti-Sheep IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Sheep
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Immunomicroscopy, Dot blotmore details
  • Immunogen

    Full length native Sheep IgG (purified).

  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Conjugation notes

    Biotin/Protein Ratio: about 6 per IgG molecule
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
IHC-P 1/1000 - 1/5000.
IHC-Fr 1/1000 - 1/5000.
WB 1/2000 - 1/10000.
ELISA 1/20000 - 1/100000.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.

References

This product has been referenced in:

  • Davis EA  et al. Long-term effect of parasympathetic or sympathetic denervation on intestinal epithelial cell proliferation and apoptosis. Exp Biol Med (Maywood) 242:1499-1507 (2017). Read more (PubMed: 28766984) »
  • Blackmore K  et al. LKB1-AMPK modulates nutrient-induced changes in the mode of division of intestinal epithelial crypt cells in mice. Exp Biol Med (Maywood) 242:1490-1498 (2017). Read more (PubMed: 28766983) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Merci pour votre appel et votre patience.

Je viens d'avoir la confirmation du laboratoire que effectivement le ratio de conjugaison de la biotine était de ˜6 moles de biotine par mole de IgG.

Je suis désolée pour cette erreur et je vous remercie d'avoir attiré notre attention à ce fait.

J'espère que cette information vous est utile. N'hésitez pas à nous recontacter si vous avez des autres questions.

Je vous souhaite un bon fin de semaine.

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Answer

Thank you for your enquiry. We have yet to receive reviews from customers who have purchased and tested this antibody in WB or IHC. We also, don't have the same product with a fluorescent conjugate. To view a full list of our secondary antibodies, you can log onto our website and use the product browser. Please contact us again if you require further information to that provided on the datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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