Key features and details
- Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (Biotin)
- Conjugation: Biotin
- Host species: Donkey
- Isotype: IgG
- Suitable for: ELISA, ICC/IF, IHC-P, WB
Product nameDonkey Anti-Sheep IgG H&L (Biotin)
See all IgG secondary antibodies
DescriptionDonkey polyclonal Secondary Antibody to Sheep IgG - H&L (Biotin)
Tested applicationsSuitable for: ELISA, ICC/IF, IHC-P, WBmore details
Other Immunogen Type corresponding to Sheep IgG.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
Conjugation notesBiotin/ antibody protein ratio is 4:1
Our Abpromise guarantee covers the use of ab97123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/20000 - 1/400000. (primary)|
|ICC/IF||1/200 - 1/1000.|
|IHC-P||1/500 - 1/5000.|
|WB||1/20000 - 1/400000. (chemiluminescent)|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97123 has been referenced in 2 publications.
- Yildiz M et al. Effects of Intracordal Estradiol and Dexamethasone Injection on Wound Healing in Vocal Fold Injuries. J Voice N/A:N/A (2018). PubMed: 29496298
- Thomasy HE et al. Hypocretinergic and cholinergic contributions to sleep-wake disturbances in a mouse model of traumatic brain injury. Neurobiol Sleep Circadian Rhythms 2:71-84 (2017). PubMed: 31236496