Overview

  • Product name

    Donkey Anti-Sheep IgG H&L (DyLight® 488)
    See all IgG secondary antibodies
  • Description

    Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (DyLight® 488)
  • Host species

    Donkey
  • Target species

    Sheep
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Conjugation

    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:

  • Cen M  et al. TIEG1 deficiency confers enhanced myocardial protection in the infarcted heart by mediating the Pten/Akt signalling pathway. Int J Mol Med 39:569-578 (2017). Read more (PubMed: 28204828) »
  • Kühn N  et al. Regulation of polar auxin transport in grapevine fruitlets (Vitis vinifera L.) and the proposed role of auxin homeostasis during fruit abscission. BMC Plant Biol 16:234 (2016). Read more (PubMed: 27793088) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

According to my records, ab139923 was proving difficult to use in IHC and we were in contact in order to help resolve the issue.



Looking at our correspondence, it appears that we are awaiting protocol details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.



If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.



We wish you the best of luck with your research and look forward to a reply.

Read More

Question
Answer

We recommend using the filter used for FITC.

Read More

Answer

Thank you for contacting Abcam regarding ab96939.


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Thank you for your reply. My colleague is not in the office today and that is the reason I am writing to you.

I ma sorry there was a misunderstanding.

We do have information on the spectra of DyLight® fluorochromes on our homepage:

https://www.abcam.com/index.html?pageconfig=resource&rid=12934

The spectra can be reviewed on the bottom of the page. Unfortunately, we do not have a spectra viewer where multiple dyes can be combined and tested for overlapping. I am sorry if this causes any inconvenience.

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. Maybe it is worth checking their website for a spectra viewer also.

I hope this information is helpful and wish you good luck with your experiments.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up