Donkey Anti-Sheep IgG H&L (DyLight® 650) (ab96942)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameDonkey Anti-Sheep IgG H&L (DyLight® 650)
See all IgG secondary antibodies -
DescriptionDonkey polyclonal Secondary Antibody to Sheep IgG - H&L (DyLight® 650)
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Host speciesDonkey
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Target speciesSheep
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SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
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Tested applicationsSuitable for: Flow Cyt, IHC-P, ICC/IFmore details
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ConjugationDyLight® 650. Ex: 654nm, Em: 673nm
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
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ClonalityPolyclonal
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IsotypeIgG
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General notesDyLight® 650 replaces DyLight® 649.
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Research areas
Applications
Our Abpromise guarantee covers the use of ab96942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| Flow Cyt | 1/50 - 1/200. | |
| IHC-P | 1/50 - 1/500. DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
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| ICC/IF | 1/50 - 1/500. |
Images
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (DyLight® 650) (ab96942)This image is courtesy of an Abreview submitted by Marcella Flores.ab62116 staining MAP1LC3A in mouse splinocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 2% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1 µg/ml in PBS/2% FBS) for 24 hour at 4°C. A DyLight® 650-conjugated donkey anti-sheep IgG H&L (ab96942) (1/1000) was used as the secondary antibody.
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DyLight®-Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (DyLight® 650)(ab96942)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
References
This product has been referenced in:
- Esch MB et al. Multi-cellular 3D human primary liver cell culture elevates metabolic activity under fluidic flow. Lab Chip 15:2269-77 (2015). Read more (PubMed: 25857666) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"