Overview

  • Product name

    Donkey Anti-Sheep IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Sheep
  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, IHC-P, WB, ELISA, Immunomicroscopy, Dot blotmore details
  • Immunogen

    Full length native Sheep IgG (purified).

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Conjugation notes

    Horseradish Peroxidase (HRP)
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
IHC-Fr 1/1000 - 1/5000.
IHC-P 1/1000 - 1/5000.
WB 1/3000 - 1/15000.
ELISA 1/150000.
Immunomicroscopy Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.

Images

  • IHC image of Histone H3 staining in human normal colon formalin fixed paraffin embedded tissue section*.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab128012, 10 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6900, 1/1000 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ab6900 was used at dilution 1/20000 with the primary antibody ab30524 in WB. See the review on ab30524.

  • ELISA results of purified ab6900 tested against purified Sheep IgG.

    Each well was coated in duplicate with 1.0 µg of Sheep IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

References

This product has been referenced in:

See all 8 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

Many of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest pipetting up and down few times,vortexing the vial and using this antibody as normal. In our retesting experiment and from customer’s feedback we can say that ab is absolutely fine to use. So I would really encourage you to use the antibody first; If in case the antibody fails please do not hesitate to contact me for further assistance.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your inquiry.

It is normal to have brown precipitate form for HRP conjugates. The antibody that remains in solution is functional and the brown pellet should be discarded.

Irecommend to spin down the vial and try the antibody from the supernatantin your experiment. You might want to increase the amount you are using a little bit.

I would like to assureyou that if one of our products is not functioning as stated on the datasheet and was purchased with the last six month we will be happy to provide a free of charge replacement of credit note.

Please let me know if the antibody does not generate successful results after removing the precipitate.

Thank you for your cooperation.

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