Overview

  • Product name

    Donkey Anti-Sheep IgG H&L (HRP)
    See all IgG secondary antibodies
  • Description

    Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (HRP)
  • Host species

    Donkey
  • Target species

    Sheep
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications

    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Immunogen

    Other Immunogen Type corresponding to Sheep IgG.

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes

    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (primary)
WB 1/5000 - 1/50000. Colorimetric: 1/500 - 1/30000, Chemiluminescent: 1/10000 - 1/50000

References

This product has been referenced in:

  • Cheng X  et al. Morphological and molecular comparisons between tibialis anterior muscle and levator veli palatini muscle: A preliminary study on their augmentation potential. Exp Ther Med 15:247-253 (2018). Read more (PubMed: 29375687) »
  • Van de Bittner GC  et al. Nasal neuron PET imaging quantifies neuron generation and degeneration. J Clin Invest 127:681-694 (2017). WB . Read more (PubMed: 28112682) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abreviews
Application
Western blot
Rat olfactory epithelium tissue lysate, 10ug/lane
Blocked overnight in 5% nonfat milk
GAP43 1/2000, 1hr in TBS+0.1%Tween
Donkey anti-sheep 1/5000 in 3.5%BSA/TBS+-.1%Tween
Lane1: GAP43 primary antibody, Lane 2: Secondary only negative control

There is a general high background to the secondary but seems to be improved with secondary incubation in BSA and extended washing after secondary incubation.

Abcam user community

Verified customer

Submitted Jul 14 2015

Answer

Gracias por su respuesta. Un anticuerpo secundario válido para usar con el primario ab64544 podría ser el ab97125: https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Sheep-IgG-H-L-HRP-ab97125.html está conjugado con peroxidasa y es apropiado para ELISA. Un sustrato apropiado para usar en ELISA con peroxidasa es 3,3',5,5'-Tetramethylbenzidine (TMB). Lamentablemente nosotros no lo comercializamos, pero le adjunto el link de una casa comercial, Thermo Scientific, que si lo comercializa, y quizás le pueda interesar: http://www.piercenet.com/browse.cfm?fldID=01041009 Espero haberle ayudado. En cuanto tenga una respuesta acerca del kit de BDNF volveré a contactarle. Mientras tanto, si necesita cualquier otra información, por favor, no dude en volvernos a contactar.

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