Key features and details
- Donkey Anti-Sheep IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: ELISA, WB, IHC
Product nameDonkey Anti-Sheep IgG H&L (HRP) preadsorbed
See all IgG secondary antibodies
Donkey Anti-Sheep IgG H&L (HRP) preadsorbed exhibits minimal cross reactivity to Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.
Tested applicationsSuitable for: ELISA, WB, IHCmore details
Full length native protein (purified) corresponding to Sheep IgG.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Our Abpromise guarantee covers the use of ab195176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/10000 - 1/50000.|
|WB||1/1000 - 1/10000.|
|IHC||1/500 - 1/2500.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab195176 has been referenced in 1 publication.
- Dengler F & Gäbel G The Fast Lane of Hypoxic Adaptation: Glucose Transport Is Modulated via A HIF-Hydroxylase-AMPK-Axis in Jejunum Epithelium. Int J Mol Sci 20:N/A (2019). PubMed: 31601024