Donkey Anti-Sheep IgG H&L (TRITC) (ab6897)
Key features and details
- Donkey Anti-Sheep IgG H&L (TRITC)
- Conjugation: TRITC. Ex: 547nm, Em: 572nm
- Host species: Donkey
- Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISA
Overview
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Product name
Donkey Anti-Sheep IgG H&L (TRITC)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Sheep -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISAmore details -
Immunogen
Full length native Sheep IgG (purified).
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Conjugation
TRITC. Ex: 547nm, Em: 572nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG -
Clonality
Polyclonal -
Research areas
Applications
Our Abpromise guarantee covers the use of ab6897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/1000 - 1/5000. PubMed: 20888852 | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent dilution. | |
| ELISA | 1/10000 - 1/50000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References
ab6897 has been referenced in 5 publications.
- Godoy-Calderón MJ et al. Autologous tumor cells/bacillus Calmette-Guérin/formalin-based novel breast cancer vaccine induces an immune antitumor response. Oncotarget 9:20222-20238 (2018). PubMed: 29755647
- Ferguson GB et al. Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes. Nat Commun 9:3634 (2018). PubMed: 30194383
- Ding XQ et al. Transient Receptor Potential Melastatin 4 (TRPM4) Contributes to High Salt Diet-Mediated Early-Stage Endothelial Injury. Cell Physiol Biochem 41:835-848 (2017). PubMed: 28214884
- Wu MM et al. Hypoxia augments the calcium-activated chloride current carried by anoctamin-1 in cardiac vascular endothelial cells of neonatal mice. Br J Pharmacol 171:3680-92 (2014). PubMed: 24758567
- D'Suze G et al. Apoptogenic peptides from Tityus discrepans scorpion venom acting against the SKBR3 breast cancer cell line. Toxicon 56:1497-505 (2010). ICC/IF . PubMed: 20888852
