Donkey Anti-Sheep IgG H&L (TRITC) (ab6897)

Submit a review Q&A (1)References (3)

Overview

  • Product name
    Donkey Anti-Sheep IgG H&L (TRITC)
    See all IgG secondary antibodies
  • Host species
    Donkey
  • Target species
    Sheep
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISAmore details
  • Immunogen

    Full length native Sheep IgG (purified).

  • Conjugation
    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000. PubMed: 20888852
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.

References

This product has been referenced in:
  • Godoy-Calderón MJ  et al. Autologous tumor cells/bacillus Calmette-Guérin/formalin-based novel breast cancer vaccine induces an immune antitumor response. Oncotarget 9:20222-20238 (2018). Read more (PubMed: 29755647) »
  • Wu MM  et al. Hypoxia augments the calcium-activated chloride current carried by anoctamin-1 in cardiac vascular endothelial cells of neonatal mice. Br J Pharmacol 171:3680-92 (2014). Read more (PubMed: 24758567) »
See all 3 Publications for this product

Publishing research using ab6897? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Our customer is looking for yellow fluroscence(ab6897), so I recommend TRITC following abcam's guide.
But, the customer insists it is red dye.
Check this please.
Best regards,

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Answer

Thank you for contacting us.

TRITC conjugated or Dylight 549 conjugated could be suggested to customer.

ab6897 is a correct product.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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