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  1. Link

    donkey-sheep-igg-hl-tritc-ab6897.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Donkey Anti-Sheep IgG H&L (TRITC) (ab6897)

  • Datasheet
Submit a review Q&A (1)References (6)

Product price, shipping and contact information

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Donkey Anti-Sheep IgG H&L (TRITC)
  • Conjugation: TRITC. Ex: 547nm, Em: 572nm
  • Host species: Donkey
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISA

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 550 DyLight® 650 FITC Gold 12nm HRP Texas Red ® Unconjugated

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Overview

  • Product name

    Donkey Anti-Sheep IgG H&L (TRITC)
    See all IgG secondary antibodies
  • Host species

    Donkey
  • Target species

    Sheep
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt, ELISAmore details
  • Immunogen

    Full length native Sheep IgG (purified).

  • Conjugation

    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG
  • Clonality

    Polyclonal
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Sheep
    • IgG
    • Fluorophore
    • Rhodamine

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/1000 - 1/5000. PubMed: 20888852
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.
Notes
ICC/IF
1/1000 - 1/5000. PubMed: 20888852
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (6)

Publishing research using ab6897? Please let us know so that we can cite the reference in this datasheet.

ab6897 has been referenced in 6 publications.

  • Härdtner C  et al. Inhibition of macrophage proliferation dominates plaque regression in response to cholesterol lowering. Basic Res Cardiol 115:78 (2020). PubMed: 33296022
  • Godoy-Calderón MJ  et al. Autologous tumor cells/bacillus Calmette-Guérin/formalin-based novel breast cancer vaccine induces an immune antitumor response. Oncotarget 9:20222-20238 (2018). PubMed: 29755647
  • Ferguson GB  et al. Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes. Nat Commun 9:3634 (2018). PubMed: 30194383
  • Ding XQ  et al. Transient Receptor Potential Melastatin 4 (TRPM4) Contributes to High Salt Diet-Mediated Early-Stage Endothelial Injury. Cell Physiol Biochem 41:835-848 (2017). PubMed: 28214884
  • Wu MM  et al. Hypoxia augments the calcium-activated chloride current carried by anoctamin-1 in cardiac vascular endothelial cells of neonatal mice. Br J Pharmacol 171:3680-92 (2014). PubMed: 24758567
  • D'Suze G  et al. Apoptogenic peptides from Tityus discrepans scorpion venom acting against the SKBR3 breast cancer cell line. Toxicon 56:1497-505 (2010). ICC/IF . PubMed: 20888852

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Our customer is looking for yellow fluroscence(ab6897), so I recommend TRITC following abcam's guide.
But, the customer insists it is red dye.
Check this please.
Best regards,

Read More

Abcam community

Verified customer

Asked on Aug 22 2012

Answer

Thank you for contacting us.

TRITC conjugated or Dylight 549 conjugated could be suggested to customer.

ab6897 is a correct product.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Aug 22 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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